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Characterization of the Quality of Imbibed Soybean at an Early Stage of Pre-Germination for the Development of a New Protein Food Item

ABSTRACT: Global analyses on gene expression profiles in short-term imbibition (0, 8, and 16 h) was performed using DNA microarray technique to look at changes of expression of genes coding for components that may determine food protein quality. DNA microarray analysis followed by gene ontology statistics revealed that, prior to 16 h, (i) genes for carbohydrate metabolism giving rise to nutrients as the primary metabolism were up-regulated; (ii) genes response to water-, temperature-, and light-stimulation which may not be related to soybean protein properties were down-regulated; and (iii) gene associated with lipid metabolism and secondary metabolite production which can be activated during germination showed indistinct up- and down-regulations.

ORGANISM(S): Glycine max

PROVIDER: GSE48913 | GEO | 2013/12/13

SECONDARY ACCESSION(S): PRJNA212298

REPOSITORIES: GEO

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Characterization of the Quality of Imbibed Soybean at an Early Stage of Pre-Ggermination for the Development of a New Protein Food Item

Project description:Global analyses on gene expression profiles in short-term imbibition (0, 8, and 16 h) was performed using DNA microarray technique to look at changes of expression of genes coding for components that may determine food protein quality. DNA microarray analysis followed by gene ontology statistics revealed that, prior to 16 h, (i) genes for carbohydrate metabolism giving rise to nutrients as the primary metabolism were up-regulated; (ii) genes response to water-, temperature-, and light-stimulation which may not be related to soybean protein properties were down-regulated; and (iii) gene associated with lipid metabolism and secondary metabolite production which can be activated during germination showed indistinct up- and down-regulations. Soybean seeds were selected at 0, 8, and 16 h imbibition for RNA extraction and hybridization on Agilent microarrays.

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Transcription profiling of Arabidopsis seed germination in presence and absence of histone deaceatylase inhibitor, Trichostain A (TSA).

Project description:Histone acetylation is involved in the regulation of gene expression in plants and eukaryotes. Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from histones, which is associated with the repression of gene expression. To study the role of histone acetylation in the regulation of gene expression during seed germination, trichostatin A (TSA), a specific inhibitor of histone deacetylase, was used to treat imbibing Arabidopsis thaliana seeds. GeneChip arrays were used to show that TSA induces up-regulation of 45 genes and down-regulation of 27 genes during seed germination. Eight TSA-up-regulated genes were selected for further analysis - RAB18, RD29B, ATEM1, HSP70 and four late embryogenesis abundant protein genes (LEA). A gene expression time course shows that these eight genes are expressed at high levels in the dry seed and repressed upon seed imbibition at an exponential rate. In the presence of TSA, the onset of repression of the eight genes is not affected but the final level of repressed expression is elevated. Chromatin immunoprecipitation and HDAC assays show that there is a transient histone deacetylation event during seed germination at one day after imbibition, which serves as a key developmental signal that affects the repression of the eight genes. Experiment Overall Design: Three samples: Unimbibed seeds, untreated imbibed seeds, TSA-treated imbibed seeds.

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