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Gene Expression Profile identify a role for cyclooxygenase 2-depedent prostinoid generation in BMP6-induced

ABSTRACT: The bone morphogenetic protein (BMP) family of proteins participates in an underappreciated signaling pathway involved in regulating angiogensis in physiological and pathological conditions. Microarray profiles of BMP-responsive genes in the endothelium performed by our lab suggest important roles for cyclooxygenase-2 (Cox2) as a potential downstream regulator of BMP-induced angiogenesis. Cox2 is upregulated by BMP6 at both mRNA and protein levels. Inhibition of Cox2 blocks BMP6 induced endothelial cell proliferation, migration and tube formation activity. Microvessel outgrowth stimulated by BMP6 also required Cox2 activity. These results strongly support that Cox2 is an important downstream target for BMP6, and that prostaniod synthesis is essential for BMP6 induced endothelial cell activation. This further suggests that our microarray data is a reliable tool to identify unkown BMP6 targets in angiogenesis. Keywords: BMP, angiogenesis, Cox2, MEC

ORGANISM(S): Mus musculus

PROVIDER: GSE4909 | GEO | 2006/12/31

SECONDARY ACCESSION(S): PRJNA95911

REPOSITORIES: GEO

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Quantitative proteomics and RNA-sequencing of mouse liver endothelial cells identify novel regulators of BMP6 by iron

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2023-12-28 | PXD039830 | Pride

A time series study on BMP6 induced osteoblast differentiation of human mesenchymal stem cells (hMSC)

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Effects of RGMc on BMP2 and BMP6-mediated Gene Expression in Hep3B Liver Cells

Project description:Mutations in repulsive guidance molecule c (RGMc) / hemojuvelin (HJV) cause juvenile hemochromatosis, an aggravated iron overload disorder that presents early in life. Patients with juvenile hemochromatosis, and RGMc knockout mice, have diminished expression of the key iron-regulatory peptide, hepcidin. This suggests that RGMc plays a critical role in the regulation of iron homeostasis; however the mechanisms of RGMc actions are unknown. Recent studies have shown that RGMc directly binds to the growth factors, bone morphogenetic protein 2 and 6 (BMP2 and BMP6), and it is possible that this interaction regulates aspects of iron metabolism. We used microarrays to examine the effects of RGMc on BMP2- and BMP6-mediated gene expression. In our experimental model we treated Hep3B liver cells that had been serum starved for 16 hours as follows: (1) un-treated, (2) BMP2, (3) BMP2 + 10-fold molar excess of Noggin (a potent BMP2 inhibitor), (4) BMP2 + 20-fold molar excess of RGMc, (5) BMP6, (6) BMP6 + 10-fold molar excess of Noggin, (7) BMP6 + 20-fold molar excess of RGMc. Binding was allowed to proceed for BMP and Noggin or RGMc for 3 hr at 20Â°C prior to treatment of Hep3B cells. Treatment time was 4 hr at which cells were collected for RNA isolation.

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2009-06-08 | E-GEOD-13604 | biostudies-arrayexpress

8 hours BMP6 treated vs untreated human mesenchymal stem cells (hMSC)

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