2D and 3D Madin-Darby Canine Kidney Cell Type II cell line response to hepatocyte growth factor
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ABSTRACT: Analysis of 2D (transwell) and 3D (collagen type I) cultured MDCK cells and HGF (a MAPK activator). Traditional 2D cultures are fast and inexpensive but do no mimic natural niche/cell environment as well as the more laborious and costly 3D-cultures. 3D cultures, arguably, are better models for the study of developmental processes, such as tubulogenesis. Epithelial organs (such as kidney) develop via tubulogenesis, a process, at least in part, regulated by MAPK signaling. Therefore, 2D and 3D cells also treated with HGF plus MAPK inhibitors. Results provide insights into differential response to HGF-induced tubulogenesis depending on cell culture conditions (2D vs. 3D).
ORGANISM(S): Canis lupus familiaris
PROVIDER: GSE49518 | GEO | 2014/03/31
SECONDARY ACCESSION(S): PRJNA214098
REPOSITORIES: GEO
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