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MiRQC data for NanoString nCounter miRNA Expression Assays.

ABSTRACT: nCounter miRNA Expression Assay data for all profiled miRQC samples prepared by the miRQC Consortium. In this study, we systematically compared 12 commercially available miRNA expression platforms by measuring an identical set of standardized positive and negative control samples, including human universal reference RNA, human brain RNA and titrations thereof, human serum samples, and synthetic spikes from miRNA family members with varying homology. We developed novel and robust quality metrics to objectively assess platform performance of very different technologies such as small RNA sequencing, RT-qPCR and (microarray) hybridization. We assessed reproducibility, sensitivity, accuracy, specificity, and concordance of differential expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE49600 | GEO | 2013/12/30

SECONDARY ACCESSION(S): PRJNA214422

REPOSITORIES: GEO

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