Hemin and Iron transcriptome of Porphyromonas gingivalis (part 2)
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ABSTRACT:
ORGANISM(S): Porphyromonas gingivalis
PROVIDER: GSE50702 | GEO | 2015/05/18
SECONDARY ACCESSION(S): PRJNA218625
REPOSITORIES: GEO
Similar Datasets
Project description:Transcriptional profiling of P. gingivalis cells comparing cells grown under hemin replete conditions to that grown under hemin depleted conditions
2015-05-18 | GSE50701 | GEO
Project description:Transcriptional profiling of P. gingivalis cells comparing cells grown under hemin replete conditions to that grown under hemin depleted conditions Two-condition experiment, hemin replete vs. hemin deplete grown cells. Biological replicates: 3 independently grown and harvested bacterial cells. Four technical replicates (four replicates per array).
2015-05-18 | E-GEOD-50701 | biostudies-arrayexpress
Project description:Wild type Porphyromonas gingivalis strain ATCC33277 (V3176) and PG1626 - deficient mutant (V3177) were grown in iron replete conditions was used to compare to Porphyromonas gingivalis strains grown in iron chelated conditions.
2023-06-08 | GSE174493 | GEO
Project description:Transcription profiling of P. gingivalis W50 grown in continuous culture under conditions of heme-excess and heme-limitation.
2008-11-19 | GSE13375 | GEO
Project description:Transcriptional profiling of P. gingivalis cells comparing control cells grown in anaerobic conditions with cells grown in the presence of 6% of oxygen
2009-11-13 | GSE17960 | GEO
Project description:Porphyromonas gingivalis is a major pathogen associated with the microbial biofilm-mediated disease chronic periodontitis. P. gingivalis has an obligate requirement for iron and protoporphyrin IX which it satisfies by transporting heme and iron liberated from the human host. The level of cellular iron in P. gingivalis affects the expression of a distinct iron-associated regulon of 64 genes and low iron invokes an iron sparing response. Iron homeostasis is usually mediated in Gram-negative bacteria at the transcriptional level by the Ferric Uptake Regulator (Fur). There is a single predicted P. gingivalis Fur superfamily orthologue named Har (heme associated regulator) that lacks the conserved metal binding residues found in other Fur orthologues. We show that Har binds both heme and ferrous iron resulting in a conformational change in the protein. Har was unable to complement the Escherichia coli H1780 fur mutant and there was no change in cellular metal content in a P. gingivalis Har mutant compared with the wild-type. The Har regulon of 44 genes is not predicted to play a role in iron homeostasis. Together these data indicated that Har does not regulate iron homeostasis in P. gingivalis. However, Har was required for heme-responsive biofilm development and its regulon overlapped P. gingivalis regulons previously identified after growth in heme limitation or as a homotypic biofilm. P. gingivalis is unique as an iron-dependent Gram-negative bacterium with a single heme-binding Fur superfamily orthologue, Har, that does not regulate iron homeostasis.
2015-04-01 | GSE37099 | GEO
Project description:Porphyromonas gingivalis is a major pathogen associated with the microbial biofilm-mediated disease chronic periodontitis. P. gingivalis has an obligate requirement for iron and protoporphyrin IX which it satisfies by transporting heme and iron liberated from the human host. The level of cellular iron in P. gingivalis affects the expression of a distinct iron-associated regulon of 64 genes and low iron invokes an iron sparing response. Iron homeostasis is usually mediated in Gram-negative bacteria at the transcriptional level by the Ferric Uptake Regulator (Fur). There is a single predicted P. gingivalis Fur superfamily orthologue named Har (heme associated regulator) that lacks the conserved metal binding residues found in other Fur orthologues. We show that Har binds both heme and ferrous iron resulting in a conformational change in the protein. Har was unable to complement the Escherichia coli H1780 fur mutant and there was no change in cellular metal content in a P. gingivalis Har mutant compared with the wild-type. The Har regulon of 44 genes is not predicted to play a role in iron homeostasis. Together these data indicated that Har does not regulate iron homeostasis in P. gingivalis. However, Har was required for heme-responsive biofilm development and its regulon overlapped P. gingivalis regulons previously identified after growth in heme limitation or as a homotypic biofilm. P. gingivalis is unique as an iron-dependent Gram-negative bacterium with a single heme-binding Fur superfamily orthologue, Har, that does not regulate iron homeostasis. Paired samples were compared on the same microarray using a two-colour system. A total of 6 paired microarray hybridizations were performed representing 6 biological replicates, where a balanced dye design was used, with the overall analysis including three microarrays where P. gingivalis 33277 samples were labeled with Cy3 and the paired ECR455 samples were labeled with Cy5 and three other microarrays where samples were labeled with the opposite combination of fluorophores.
2015-04-01 | E-GEOD-37099 | biostudies-arrayexpress
Project description:This SuperSeries is composed of the SubSeries listed below.
2015-05-18 | GSE50703 | GEO
Project description:Transcriptional profiling of P. gingivalis cells comparing cells grown in anaerobic conditions to cells grown in anaerobic conditions and exposed to nitrite and to GSNO.
2012-10-05 | GSE38220 | GEO
Project description:Extracytoplasmic function (ECF) sigma factors are known to play an important role in the bacterial response to various environmental stresses. Porphyromonas gingivalis, a prominent etiological agent in human periodontitis, possesses six putative ECF sigma factors. So far, information is limited on an ECF sigma factor, PGN_0319. It has been recently reported that the expression of genes encoding some of the ECF sigma factors including PGN_0319 was affected by hemin availability. The aim of this study was to evaluate the role of PGN_0319 in hemin utilization by P. gingivalis. We evaluated the gene expression profile of the PGN_0319 mutant by DNA microarray, and then real-time reverse transcription PCR analysis was performed to assess genes with altered expression levels. The expressions level of hmuY and hmuR, which encode an outer-membrane protein involved in hemin utilization, and cdhR, a transcriptional regulator of hmuYR, were significantly decreased in the PGN_0319 mutant. The transcription of these genes was restored in the PGN_0319 complemented strain. We observed that the PGN_0319 mutant showed reduced growth in log phase compared with wild type under hemin-limiting condition. By electrophoretic mobility shift assay we demonstrated the recombinant PGN_0319 protein bound to the promoter region of hmuY and cdhR. In addition, we observed that PGN_0319 gene was upregulated in response to low cell density. These results demonstrate that PGN_0319 play an important role in the early growth of P. gingivalis, and directly regulates hmuYR and cdhR, thereby plays a role in the mechanisms involved in hemin utilization by P. gingivalis.
2017-01-01 | GSE78048 | GEO