Project description:Response of pancreas cancer cells to treatment with recombinant MMP3 Cells were treated with 100U/ml MMP3 for 4 days, then isolated for RNA. The submitted experiments represent two different experiments performed at different times
Project description:Response of mouse mammary epithelial cells to treatment with MMP3 Cells were untreated (d1 control) or treated with MMP3 for 1-4 days (d1-4 MMP3), or washed and then allowed to recover for three additional days (d5-7 recover) then isolated for RNA.
Project description:Response of pancreas cancer cells to treatment with recombinant MMP3 Cells were treated with 100U/ml MMP3 for 4 days, then isolated for RNA. The submitted experiments represent two different experiments performed at different times
Project description:Cancer metastasis remains an important unsolved problem. Matrix metalloproteinases (MMPs) have been shown to promote cancer cell transformation, migration, invasion, and metastasis through alteration of the extracellular microenvironment, and alter intracellular signaling and genome status. In addition, recent studies have shown intracellular and intranuclear localization, as well as roles of MMPs. In the present study, we examined gene expression signatures of high- and low-metastatic mouse colon cancer cells, and found that Mmp3 was expressed at the highest level in the high-metastatic cells. Profound nuclear localization of Mmps was found in primary explant sites as well as in areas of metastasis in lungs. In addition to the native 50-kDa Mmp3, a short 25-kDa PEX domain and active Mmp3 dimer were found in metastatic cancer cells, indicating novel roles for these forms. Knockdown of Mmp3 attenuated cancer cell viability, migration, and invasion in vitro, along with metastasis in an in vivo transplantation model, as well as cancer cell migration and invasion. These findings suggest that MMPs including intracellular, short, and dimerized forms are involved with malignant progression of cancer, thus they may be suitable as biomarkers and therapeutic targets.
Project description:Effect of induction of MMP3 expression in CCSP-rtTA/tet-MMP3 transgenic mice, fed doxycycline for 14 weeks We used microarrays to detail the global programme of gene expression following MMP3 expression Transgenic mice (CCSPrt-TA/tet-MMP3 or CCSPrtTA control mice) were fed doxycycline from week 6 to week 20, then lungs were removed, formalin fixed, and embedded in paraffin. Sections were sliced from the paraffin blocks for RNA extraction.
Project description:Effect of induction of MMP3 expression in CCSP-rtTA/tet-MMP3 transgenic mice, fed doxycycline for 14 weeks We used microarrays to detail the global programme of gene expression following MMP3 expression
Project description:Molecular comparison between control 4T1 cells with MMP3-low 4T1 cells RNA extracted from biological triplicates of each of the above mentioned cell populations were subjected to microarray analysis