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Deletion of HCMV US17 alters host immune genes at 12 hpi and ER stress genes at 96 hpi.

ABSTRACT: Infection of human fibroblasts with either human cytomegalovirus strain AD169 or a recombinant virus deleted for HCMV US17 Illumina HT-12 beadarrays were used to quantitate levels of host transcripts at either 12 or 96 hpi.

ORGANISM(S): Homo sapiens

PROVIDER: GSE50955 | GEO | 2013/11/30

SECONDARY ACCESSION(S): PRJNA219423

REPOSITORIES: GEO

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Deletion of HCMV US17 alters host immune genes at 12 hpi and ER stress genes at 96 hpi.

Project description:Infection of human fibroblasts with either human cytomegalovirus strain AD169 or a recombinant virus deleted for HCMV US17 Illumina HT-12 beadarrays were used to quantitate levels of host transcripts at either 12 or 96 hpi. Cells were infected in biological triplicates at both 12 and 96 hours post infetion

2013-11-30 | E-GEOD-50955 | biostudies-arrayexpress

HCMV infected Human Fibroblasts UL84 IE2 ChIPSeq

Project description:We report ChIP-seq analsyis of human fibroblasts infected with HCMV strains AD169 and TB40E. ChIP was performed at 20 hpi for IE2 and 3 dpi for IE2 and UL84.

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RNA-seq transcriptome analysis of HCMV (AD169) and mock infected MRC5 fibroblasts

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Transcription profiling of human neural stem cell culture after infection with HCMV reveals HCMV infection disrupts the self-renewal capacity of neural progenitor cells and inhibits neuronal differentiation

Project description:Our results suggest that HCMV infection disrupts the self-renewal capacity of NPCs and influences their differentiation. Whole genome expression analysis revealed many changes in cellular gene expression, including downregulation of genes pertinent to the neuronal lineage. Experiment Overall Design: For gene expression analysis, cells were either mock- or virus-infected. Mock-infectedÂ cells were harvested at 12 hpi, virus infected cells were harvested at 4, 12 and 24 hpi. Virus- and mock-infections were performed in duplicate, with each individual sample harvested separately, thereby acting as biological replicates for analysis.

2009-12-14 | E-GEOD-19345 | biostudies-arrayexpress

RNA-seq Transcriptome Analysis of AD169 and AD169-ΔUL26 infected MRC5 fibroblasts.

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Pol2 occupancy of the viral and host genomes in HCMV infected Kasumi-3 cells at 24 hpi

Project description:Pol2 occupancy of the viral and host genomes in HCMV infected Kasumi-3 cells at 24 hpi

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Kinome profiling upon HCMV infection using the MIB-MS approach

Project description:Human cytomegalovirus (HCMV) is a significant cause of disease in immune-compromised adults and immune naïve newborns. No vaccine exists to prevent HCMV infection, and current antiviral therapies have toxic side effects that limit the duration and intensity of their use. There is thus an urgent need for new strategies to treat HCMV and repurposing existing drugs as antivirals is an attractive approach. Virus-induced changes in infected cells are often driven by changes in cellular kinase activity, leading us to hypothesize that defining the complement of kinases (the kinome), whose activity or expression is altered during infection would identify existing kinase inhibitors that could be repurposed as new antivirals. To this end, we applied a recently developed technique, MIB-MS kinome profiling, to quantitatively measure perturbations in >240 cellular kinases simultaneously in cells infected with a laboratory-adapted (AD169) or clinical (TB40E) HCMV strain using a label-free approach. Significant time-dependent changes for multiple kinases including cell cycle, receptor tyrosine and mitotic kinases were observed. Based on these data, we tested antiviral activity of 14 kinase inhibitors, and the most potent inhibitor blocked HCMV early gene expression and viral DNA accumulation. These results show the utility of kinome profiling to screen kinase inhibitors that can be repurposed as antivirals.

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RNA-seq transcriptome analysis of HCMV (AD169) and mock infected MRC5 fibroblasts

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Human induced pluripotent stem cell-technology to study the effects of human cytomegalovirus infection on neural differentiation

Project description:Congenital human cytomegalovirus (HCMV) infection is one of the leading prenatal causes of mental retardation and congenital deformities world-wide. Access to cultured human neuronal lineages, necessary to understand the species specific pathogenic effects of HCMV has been limited by difficulties in sustaining primary cultures. Neuronal cells derived from human induced pluripotent stem (iPS) cells now provide a novel opportunity to investigate HCMV pathogenesis. We derived iPS cells from human adult fibroblasts and induced neural lineages to investigate their permissiveness to infection with HCMV strain Ad169. Analysis of iPS cells and nearly pure populations of iPS-derived neural stem cells (NSCs), neuroprogenitor cells (NPCs) and neurons suggests that (i) iPS cells are not permissive to HCMV infection; (ii) Neural stem cells have impaired differentiation when infected by HCMV; (iii) NPCs are fully permissive for HCMV infection; the supernatant from infected neural stem cells and NPCs (but not mock infected cells) induced cytopathic effects in human fibroblasts; (iv) most iPS-derived neurons are not permissive to HCMV infection; and (v) infected neurons have impaired calcium influx in response to glutamate. Our approach offers powerful cellular models to investigate the effect of neurotropic viral agents on human neurodevelopment. Adherent monolayer culture of neural progenitor cells (NPCs) were either infected with HCMV Ad169 in triplicate, with each individual sample harvested separately to provide biological replicates for expression analysis. Infected and mock-infected cells were harvested 24 h p.i. RNA. NPCs were 70-80% confluence.

2013-01-04 | E-GEOD-35295 | biostudies-arrayexpress

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