Transcriptomics

Dataset Information

0

An Integrated Approach Reveals Regulatory Controls on Bacterial Translation Elongation


ABSTRACT: Ribosomes elongate at a nonuniform rate during translation. Theoretical models and experiments disagree on the in vivo determinants of elongation rate and the mechanism by which elongation rate affects protein levels. To resolve this conflict, we measured transcriptome-wide ribosome occupancy under multiple conditions and used it to formulate a whole-cell model of translation in E. coli. Our model predicts that elongation rates at most codons during nutrient-rich growth are not limited by the intracellular concentrations of aminoacyl-tRNAs. However, elongation pausing during starvation for single amino acids is highly sensitive to the kinetics of tRNA aminoacylation. We further show that translation abortion upon pausing accounts for the observed ribosome occupancy along mRNAs during starvation. Abortion reduces global protein synthesis, but it enhances the translation of a subset of mRNAs. These results suggest a regulatory role for aminoacylation and abortion during stress, and our study provides an experimentally constrained framework for modeling translation.

ORGANISM(S): Escherichia coli BW25113 Escherichia coli str. K-12 substr. MG1655

PROVIDER: GSE51052 | GEO | 2014/05/15

SECONDARY ACCESSION(S): PRJNA219750

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2014-05-15 | E-GEOD-51052 | biostudies-arrayexpress
2020-06-18 | GSE134541 | GEO
2011-11-12 | E-GEOD-25626 | biostudies-arrayexpress
2020-10-26 | GSE157902 | GEO
2009-02-12 | E-GEOD-13750 | biostudies-arrayexpress
2011-11-12 | E-GEOD-25331 | biostudies-arrayexpress
2016-01-13 | E-GEOD-72899 | biostudies-arrayexpress
2011-11-12 | GSE25626 | GEO
2011-11-12 | GSE25331 | GEO
2023-03-01 | GSE184825 | GEO