Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Real-time quantitative PCR analysis of human colon cancer cells for UPR related genes

ABSTRACT: human colon cancer cell line HT29 cells treated with 0.5μM thapsigargin 2.5hours. Compared with equal amount total RNA from nontreated control cells. qPCR gene expression profiling.

ORGANISM(S): Homo sapiens

PROVIDER: GSE51140 | GEO | 2013/09/25

SECONDARY ACCESSION(S): PRJNA221283

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Real-time quantitative PCR analysis of human colon cancer cells for UPR related genes

Project description:human colon cancer cell line HT29 cells treated with 0.5?M thapsigargin 2.5hours. Compared with equal amount total RNA from nontreated control cells. qPCR gene expression profiling. one sample

2013-09-25 | E-GEOD-51140 | biostudies-arrayexpress

Real-time quantitative PCR analysis of human colon cancer cells for autophagy related genes

Project description:human colon cancer cell line HT29 cells treated with 0.5μM thapsigargin 2.5hours. Compared with equal amount total RNA from nontreated control cells. qPCR gene expression profiling. one sample

2013-09-25 | GSE51141 | GEO

Real-time quantitative PCR analysis of human colon cancer cells for autophagy related genes

2013-09-25 | E-GEOD-51141 | biostudies-arrayexpress

Real-time quantitative PCR miRNA analysis of human hepatocytes

Project description:Primary human hepatocytes were treated with compounds modulating steatosis: palmitic acid, compound C and metformin qPCR miRNA expression profiling. Hepatocytes were treated as indicated in the summary. Equal amount total RNA was pooled prior to miRNA expression analysis

2020-02-11 | GSE145039 | GEO

Real-time quantitative PCR analysis of human EBV-B cells during UPR

Project description:Human B cells were isolated from peripheral blood from one healthy donor and two patients with Common Variable Immunodeficiency (CVID) and were immortalized by infection with Epstein Barr virus (EBV) in vitro. Immortalized EBV-B cells (in biological triplicates) were treated twelve different ways for 4 hours: 1.untreated control, 2. Thapsigargin (Tg) 1 µM, 3. Tunicamycin (Tm) 10 µg/ml, 4. 4-phenylbutyrate (4-PBA) 1 mM, 5. Tg 1 µM plus 4-PBA 1 mM, 6. Tm 10 µg/ml plus 4-PBA 1 mM, 7. Dimethyl sulfoxide (DMSO) 1 mM, 8. Tg 1 µM plus DMSO 1 mM, 9. Tm 10 µg/ml plus DMSO 1 mM, 10. Tauroursodeoxycholic acid (TUDCA) 5 mM, 11. Tg 1 µM plus TUDCA 5 mM, and 12. Tm 10 µg/ml plus TUDCA 5 mM. We used Applied Biosystems TaqMan OpenArray Real-Time PCR System (Thermo Scientific) to quantitate gene expression of relevant genes from the cells (in technical triplicates).

2020-01-19 | GSE126201 | GEO

Real-time quantitative PCR analysis of mouse RAW264.7 macrophages

Project description:Mouse RAW264.7 macrophages were treated with LPS, IFNb, poly(rI:rC), poly(dA:dT), VSV, HSV, Sendai virus. Genes identified by Human Innate Immunity Interactome for type I Interferon (HI5) were examined for expression. qPCR gene expression profiling. RAW264.7 macrophages were used and treated separately as indicated in the summary. Equal amount total RNA from each group was used for gene expression analysis.

2011-09-08 | E-GEOD-31790 | biostudies-arrayexpress

Real-time quantitative PCR analysis of human plasma miRNA expresson

Project description:Plasma samples from 10 colonrectal cancer patients (CRCs) (including 5 stage II and 5 stage III patients) and 10 normal controls.An amount of 5 to 10 milliliters of whole blood were obtained from each participant.The plasma was obtained by centrifugation at 1200g for 10min at 4°C.To complete the removal of residual cellular components, plasma samples were recentrifuged at 12,000g for a further 10min at 4°C.A volume of 600?L of each plasma samples from CRC group or normal control group was picked out and uniformly mixed. qPCR miRNA expression profiling. Plasma samples form 10 colonrectal cancer patients(CRCs) (including 5 stage II and 5 stage III patients) and 10 normal controls were used and treated as indicated in the summary. Equal amount total RNA from each donor was pooled prior to gene expression analysis.

2012-12-29 | E-GEOD-38716 | biostudies-arrayexpress

Real-time quantitative PCR analysis of Hepatic cell lines infected with HCV

Project description:huh7.5 hepatic cell lines were infected with HCV. RNA and Protein were extract after 72 hours and The Human Adipogenesis RTM-BM-2 ProfilerM-bM-^DM-" PCR ArrayM-BM- was used to compare the gene expression in the two group. qPCR gene expression profiling. HCV infected hepatic cells vs uninfected hepatocyte. Equal amount total RNA from each samples were used

2014-03-07 | E-GEOD-55677 | biostudies-arrayexpress

miRNA Real-time quantitative PCR analysis of EAE CD4+T cells

Project description:EAE is a mouse model of human multiple sclerosis. We used miRNA low density array to screen abnormally expressed miRNAs in autoimmune CD4+T cells.Generally, splenic CD4+T cells were isolated from three groups: healthy C57BL/6 mice, C57BL/6 mice with the induction of EAE for 16 days, and C57BL/6 mice with the induction of EAE for 32 days. Then we did miRNA profilings on these samples. Splenic CD4+T cells were isolated from the above three groups. Each group consist of six mice. Equal amount total RNA from each mouse was pooled prior to miRNA expression analysis.

2015-05-08 | E-GEOD-66621 | biostudies-arrayexpress

Real-time quantitative PCR analysis of BMI1-silenced human prostate cancer cells

Project description:BMI1-silenced Prostate cancer cells exhibited a significant change in the expression of several proliferation-associated genes. These included Cyclin-D1, BCL2, IL and NF-kappaB. An increased expression of p16, p15 and TIMP3 in BMI1-silenced tumor cells was observed List of Selected Genes Modulated by BMI1-supression in Prostate cancer Cells qPCR gene expression profiling. LNCaP and BMI1-silenced LNCaP cells were used as indicated in the summary. Equal amount of total RNA from each group was pooled prior to gene expression analysis.

2013-02-05 | E-GEOD-44049 | biostudies-arrayexpress