Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MicroRNA expression in the glomerulus of C57BL/6 mice and B6.MRLc1 glomerulonephritis mice

ABSTRACT: In isolated glomeruli by beads-perfusion methods, microRNA (miRNA) expression was analyzed in healthy C57BL/6 and B6.MRLc1 glomerulonephritis mice. The expression of 1,135 miRNAs was examined, and up-regulated or down-regulated miRNA was determined. These results provide a basical information of molecular pathology in glomerulonephritis.

ORGANISM(S): Mus musculus

PROVIDER: GSE51209 | GEO | 2014/10/01

SECONDARY ACCESSION(S): PRJNA221629

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

MicroRNA expression in the glomerulus of C57BL/6 mice and B6.MRLc1 glomerulonephritis mice

Project description:In isolated glomeruli by beads-perfusion methods, microRNA (miRNA) expression was analyzed in healthy C57BL/6 and B6.MRLc1 glomerulonephritis mice. The expression of 1,135 miRNAs was examined, and up-regulated or down-regulated miRNA was determined. These results provide a basical information of molecular pathology in glomerulonephritis. The glomeruli were collected from female C57BL/6 (n=3, 9-month-old, healthy control) and female B6.MRLc1 (n=3, 14-month-old, early stage of glomerulonephritis; n=3, 9-month-old, late stage of glomerulonephritis). The total RNA sample was purified, and total 1,135 microRNA expression was compared among healthy, early stage, and late stage.

2014-10-01 | E-GEOD-51209 | biostudies-arrayexpress

Glomerulonephritis in BXSB/MpJ-Yaa mice

Project description:Analysis of glomeruli isolated from kidneys of 4 month male BXSB/MpJ-Yaa. The BXSB/MpJ-Yaa mouse strain is a lupus-prone model. Results provide insight into the pathogenic mechanisms linked to glomerulonephritis in BXSB/MpJ-Yaa mice. The glomeruli were isolated from 6 BXSB/MpJ-Yaa (glomerulonephritis model) and BXSB/MpJ-Yaa+ (control). 6 glomerular populations of each strain were devided into 3 groups (1 group / 2 populations). 3 glomerulonephritis models versus 3 controls analysis was performed. BXSB/MpJ-Yaa is a recombinant inbred strain developed originally from a C57BL/6 female mouse and a SB/Le male mouse. Yaa (Y-linked autoimmune accelerator locus) localizing on the Y chromosome of BXSB/MpJ-Yaa male mice is the result of a duplication of an about 4 Mbp telomeric segment near the pseudoautosomal region of the X chromosome onto the Y chromosome. BXSB/MpJ-Yaa+ (control) male mice carry the wild-type Y chromosome in place of the mutant Yaa-containing Y chromosome of BXSB/MpJ-Yaa male mice.

2014-01-01 | E-GEOD-51230 | biostudies-arrayexpress

Glomerulonephritis in BXSB/MpJ-Yaa mice

2014-01-01 | GSE51230 | GEO

Digital spatial profiling revealed the mechanism involved in the progression of affected glomeruli in ANCA associated glomerulonephritis [GEOmx]

Project description:We previously showed that the rupture of Bowman’s capsule (BC) promotes the progression of crescent glomerulonephritis by enhancing CD8+ T cell entry into the glomeruli. In the present study, we utilized digital spatial profiling to simultaneously profile the altered mRNA transcript and protein abundances in the glomerular and periglomerular areas of biopsy samples of ANCA-associated glomerulonephritis. The paraffin-embedded biopsy samples were stained with Collagen IV, CD45, and Syto13 to distinguish the glomeruli with crescent formation but intact BC, with focal BC rupture, and with extensive rupture of BC and glomeruli without crescent formation and leukocytes infiltration in ANCA-GN. By assessing multiple discrete glomerular areas, we found that the transcript expression level of secreted phosphoprotein 1and its receptor CD44 were upregulated significantly in the glomeruli with more severe ruptures of BC, and their expression levels correlated positively with the fibrotic markers. We also found that 1) both alternative and classic complement pathways were activated in the glomeruli from patients with ANCA-GN; 2) M1 macrophages involved mostly in the early stage of BC rupture while M2 macrophages were involved in the late stage and may contribute to the fibrosis process of the crescents, and 3) apoptosis is likely the mechanism mediating the loss of glomerular cells in ANCA-GN. Our results demonstrate that digital spatial profiling allows the comparative analysis of mRNA and protein profiles in the individual glomeruli affected differently by the disease processes and to identify potential novel mechanisms in patients with ANCA-GN.

2022-02-01 | GSE192995 | GEO

Digital spatial profiling revealed the mechanism involved in the progression of affected glomeruli in ANCA associated glomerulonephritis [RNA-Seq]

2022-02-01 | GSE192994 | GEO

Single-cell TCR-sequencing of T cells in crescentic glomerulonephritis

Project description:Single-cell TCR sequencing of T cells from ANCA-associated glomerulonephritis patients and experimental crescentic glomerulonephritis mouse model.

2023-03-31 | GSE223107 | GEO

Gene expression data from teratomas formed by C57BL/6 (B6) ES and EiPS cells in B6 mice

Project description:Embryonic fibroblast from C57BL/6 (B6) mice were reprogrammed to EiPS without exogenous DNA integration using an single episomal vector. The EiPS cells and B6 ES cells were then transplanted into B6 mice to form teratomas. We used microarrays to profile the gene expression differences between the teratomas formed by B6 ES cells and EiPS cells.

2011-04-13 | GSE28573 | GEO

Glomerular transcriptomic analysis of the influence of Genetic background effect during anti-GBM glomerulonephritis in mice

Project description:We analyzed the impact of the genetic background during experimental passive non-accelerated anti-glomerular basement membrane glomerulonephritis (anti-GBM-GN) (an equivalent to nephrotoxic nephritis) in two different mouse genetic backgrounds (C57BL6/J vs 129S2svPAS/crl). Glomerular extracts have been made at an early time point (e.g day 4 of the induction of the glomerulonephritis). RNA have been extracted following standard procedures.

2014-07-14 | GSE56236 | GEO

miRNA expression profiling of isolated populations of prepachytene spermatocytes, pachytene spermatocytes and spermatids of PWD and B6 male mice

Project description:MiRNA expression profiling of isolated populations of prepachytene spermatocytes (LP), pachytene spermatocytes (RP) and spermatids (ST) from PWD and B6 was performed to study the genome wide variation in miRNA expression between two mouse subspecies.

2014-06-01 | GSE49443 | GEO

Raoultella ornithinolytica B6 strain:B6

Project description:Raoultella ornithinolytica B6 strain:B6 Genome sequencing

| PRJNA187030 | ENA