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Gene Expression in wild type and CCR5 knockout mice with lung metastasis

ABSTRACT: We have shown that C57BL/6J CCR5 knockout mice develop 30.4% ± 8.6% fewer B16 F10 lung nodules compared to wild type mice after the intravenous injection of 100,000 B16 F10 cells. We sought to understand this phenomenon by comparing gene expression in the lungs of these mice at 6, 24, and 48 hours after tumor injection.

ORGANISM(S): Mus musculus

PROVIDER: GSE51422 | GEO | 2013/10/25

SECONDARY ACCESSION(S): PRJNA224557

REPOSITORIES: GEO

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Gene Expression in wild type and CCR5 knockout mice with lung metastasis

Project description:We have shown that C57BL/6J CCR5 knockout mice develop 30.4% ± 8.6% fewer B16 F10 lung nodules compared to wild type mice after the intravenous injection of 100,000 B16 F10 cells. We sought to understand this phenomenon by comparing gene expression in the lungs of these mice at 6, 24, and 48 hours after tumor injection. There were 6 groups or conditions (CCR5 - 6 hours, WT - 6 hours, CCR5 - 24 hours, WT - 24 hours, CCR5 - 48 hours, WT - 48 hour); each group had 4 to 5 mice. All of the mice were injected with 100,000 B16 F10 melanoma cells by tail vein. At the designated time, the mice were anesthetized with Avertin and their lungs were perfused with PBS. After this, the lungs were harvested and snap frozen in liquid nitrogen. They were kept at minus 80 degrees C until processing. mRNA was extracted as described below and equivalent amounts of mRNA (by weight) was pooled by group.

2013-10-25 | E-GEOD-51422 | biostudies-arrayexpress

proteome analysis of Lung tissue of mice bearing B16-F10-luc-G5 melanoma tumor with sleep fragmentation and with or with out the asdmistration of GL-pp

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Impact of sevoflurane priming on B16F10 transcriptome after in vivo growth

Project description:Murine skin melanoma cell line B16-F10 (ATCC no. CRL-6475) was obtained from the national standard repository (LGC Standards, Wesel, Germany). Priming of B16-F10 cells with sevoflurane was accomplished within a closed hypoxia chamber located in an 37ºC heating cabinet. Cell flasks were put into the chamber and the chamber was flushed with 2% sevoflurane (Baxter, Unterschleissheim, Germany) in normal air with 21% O2 for 30min, followed by an incubation for additional 2h. Subsequently, cells were prepared and injected into adult male C57BL/6 mice within 2h. Processing of the cells before injection was as described above. After a maximum of 4 weeks after injection, animals were euthanized and the tumor was resected for further analysis.

2020-05-15 | GSE135690 | GEO

Metastasis Ingesting Macrophages

Project description:Comparison of Macrophages from lungs 24hrs post-injection with B16-F10 Melanoma Cells expressing the Fluoresence Protein ZsGreen. ZsGreen+ and ZsGreen- macrophages were sorted and transcriptome compared.

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Gene expression profile of NK cells from Wilde type C57BL/6 (B6) and Irf4-/- injected with B16-F10 post 20 days or not [bulk RNA-seq]

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NanoString Analysis of Gene Expression in B16.F10 Tumors Treated with the cGAS Activator, NanoISD

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Differential Gene expression in CCR5+ versus CCR5- OT-I T cells

Project description:OT-I T cells were exposed to CpG ODN-activated CCR5ko Lymph nodes for 6 h, stained for surface CCR5 and FACS-sorted into CCR5+ and CCR5- fractions In the study presented here freshly isolated OT1 T cells were exposed for 6h at 37oC to teased-out CpG ODN-activated CCR5ko LNs in 96-well plates and stained for surface CCR5 before sorting into CCR5+ (Hua2) and CCR5- (Hua1) fractions. High quality total RNA isolated from each cell fraction using TRIZOL and Qiagen RNEasy kit per manufacture's recommendation was subjected to microarray analysis using the Affymetrix Mouse Gene ST 1.0 array chip to discover differentially expressed genes.

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