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Transcriptional analysis between Streptomyces clavuligerus ATCC 27064 and mutants altered in the antibiotics production

ABSTRACT: The objective is to analyze the differential expression between the wild strain and a ccaR-deleted and oppA2::aph mutants

ORGANISM(S): Streptomyces clavuligerus

PROVIDER: GSE51435 | GEO | 2014/07/01

SECONDARY ACCESSION(S): PRJNA223169

REPOSITORIES: GEO

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Transcriptional analysis between Streptomyces clavuligerus ATCC 27064 and mutants altered in the antibiotics production

Project description:The objective is to analyze the differential expression between the wild strain and a ccaR-deleted and oppA2::aph mutants 6 biological conditions were used, three strains in two times (exponential and stationary growth phase; Streptomyces clavuligerus ATCC 27064, S. clavuligerus M-bM-^HM-^FccaR and S. clavuligerus oppA2::aph). Four biological replicates were made for each condition

2014-07-01 | E-GEOD-51435 | biostudies-arrayexpress

Transcriptional analysis between Streptomyces clavuligerus ATCC 27064 and a mutant altered in antibiotic production

Project description:The objective was to analyze the differential expression between the wild strain and the Streptomyces clavuligerus ΔclaR::aac mutant

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Transcriptional analysis between Streptomyces clavuligerus ATCC 27064 and a mutant altered in antibiotic production

Project description:The objective was to analyze the differential expression between the wild strain and the Streptomyces clavuligerus ΔclaR::aac mutant Six experimental conditions were assayed, two strains (Streptomyces clavuligerus ATCC 27064, S. clavuligerus ΔclaR::aac) in three culture times (22.5h, 46.5h and 60 h). Two biological replicates for each condition.

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Transcriptional analysis between Streptomyces clavuligerus ATCC 27064 and a pSCL4- S. clavuligerus mutant

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Streptomyces clavuligerus Transcriptome using adpA deleted and overexpressed mutants

Project description:RNA seq analysis was performed using adpA-deleted and adpA-overexpressed Streptomyces clavuligerus strains to determine its regulatory effect on tunicamycin and other secondary metabolites production

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Clavulanic acid production by the MMS 150 mutant obtained from wild type Streptomyces clavuligerus ATCC 27064.

Project description:Clavulanic acid (CA) is a powerful inhibitor of the beta-lactamases, enzymes produced by bacteria resistants to penicillin and cefalosporin. This molecule is produced industrially by strains of Streptomyces clavuligerus in complex media which carbon and nitrogen resources are supplied by inexpensive compounds still providing high productivity. The genetic production improvement using physical and chemical mutagenic agents is an important strategy in programs of industrial production development of bioactive metabolites. However, parental strains are susceptible to loss of their original productivity due genetic instability phenomenona. In this work, some S. clavuligerus mutant strains obtained by treatment with UV light and with MMS are compared with the wild type (Streptomyces clavuligerus ATCC 27064). The results indicated that the random mutations originated some strains with different phenotypes, most divergent demonstrated by the mutants strains named AC116, MMS 150 and MMS 54, that exhibited lack of pigmentation in their mature spores. Also, the strain MMS 150 presented a larger production of CA when cultivated in semi-synthetics media. Using other media, the wild type strain obtained a larger CA production. Besides, using the modifed complex media the MMS 150 strain showed changes in its lipolitic activity and a larger production of CA. The studies also allowed finding the best conditions for a lipase activity exhibited by wild type S. clavuligerus and the MMS150 mutant.

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Transcriptional analysis between Streptomyces clavuligerus::pIB139 and S. clavuligerus::pimM

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Streptomyces clavuligerus

Project description:Transcriptional analysis of a S. clavuligerus oppA2-deleted mutant

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Project description:The objective is to analyze the expression level of a Streptomyces clavuligerus oppA2-deleted mutant Experiment type Expression profiling by array

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Project description:Streptomyces clavuligerus Transcriptome using adpA deleted and overexpressed mutants

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