Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression analysis of E. coli DH1 after exposure with {C2mim]Cl

ABSTRACT: Whole genome gene expression level changes in E. coli DH1 exposed to 150 mM [C2mim]Cl in mid exponential growth, and compared to a non-exposed strain.

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 Escherichia coli DH1

PROVIDER: GSE51731 | GEO | 2014/05/01

SECONDARY ACCESSION(S): PRJNA224734

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression analysis of E. coli DH1 after exposure with {C2mim]Cl

Project description:Whole genome gene expression level changes in E. coli DH1 exposed to 150 mM [C2mim]Cl in mid exponential growth, and compared to a non-exposed strain. Total RNA was isolated from three biological replicates for each condition: 150 mM [C2mim]Cl exposed at OD(600nm) 0.6, after 30 and 60 min, no exposure to [C2mim]Cl at OD(600 nm) 0.6 after 30 and 60 min.

2014-05-01 | E-GEOD-51731 | biostudies-arrayexpress

Study of 1,4 Butanediol response in Escherichia coli DH1

Project description:Escherichia coli DH1 cultures with treated with 6% 1,4 Butanediol for 1 hour and compared with untreated cultures The data from this experiment was used to identify a candidate for further study as described in Szmidt et al 2013 Utilizing a highly responsive gene, yhjX, in E. coli based production of 1,4-Butanediol submitted to Chemical Engineering Science 4x72k E.coli gene expression microarrays were used to study the genes that are differentialy expressed in the strain DH1 grown in defined medoin and exposed to 6% 1,4 Butanediol for one hour at mid-log growth stage.

2014-03-04 | E-GEOD-55496 | biostudies-arrayexpress

E. coli BW25113 azide treated vs untreated cells

Project description:Transcriptional profiling of mid-log phase (OD600 ~ 0.8) E. coli BW25113 cells grown treated with 2 mM NaN3 for 10 minutes

2017-08-08 | GSE102322 | GEO

Escherichia coli global gene expression in urine from women with urinary tract infection

Project description:Examination of E. coli transcripts present in bacteria in urine samples from 8 patients attending a urology clinic with symptoms of cystitis, as compared to transcripts present in the same E. coli strains during mid-exponential growth in filter-sterilized human urine in vitro.

2010-11-12 | GSE24478 | GEO

Gene expression profiles in the bovine corpus luteum (CL) of the estrous cycle and pregnancy: Possible roles of chemokines in regulating CL function during pregnancy

2014-08-10 | GSE59770 | GEO

Investigating the impact of heterologous expression of the Micrococcus luteus FabH on global transcription in Escherichia coli

Project description:Investigation of whole genome gene expression level changes in a E. coli fatty acid overproducing strain with or without heterologous expression of the M. luteus FabH. The strain expressing M. luteus FabH produces more methyl ketones. This study will be further described in Goh, E.B., E.E.K. Baidoo, J.D. Keasling, and H.R. Beller. Engineering of bacterial methyl ketone synthesis for biofuels. A 11 microarray study using total RNA recovered from six separate control cultures of Escherichia coli K-12 DH1 fatty acid overproducing strain with empty vector and five separate cultures of test strain, Escherichia coli K-12 DH1 fatty acid overproducing strain with vector overexpressing M. luteus FabH. Each chip measures the expression level of 4,254 genes from Escherichia coli K-12 with eight 60-mer probe pairs (PM/MM) per gene, with 2-fold technical redundancy.

2012-01-26 | E-GEOD-32561 | biostudies-arrayexpress

Nitrogen nutrition of E. coli in the mammalian intestine

Project description:Our focus of this study is to determine the differential expression of genes related to uptake and degradation of nitrogenous compounds in E. coli colonizing the mouse intestine as compared to E. coli grown in minimal medium in vitro. We colonized CD-1 male mice with E. coli MG1655 StrR wild type and extracted the total RNA from mouse cecal mucus (GSE217743). We also colonized CD-1 male mice with E. coli MG1655 ∆glnG StrR CamR and extracted the total RNA from mouse cecal mucus. We grew E. coli MG1655 StrR wild type in MOPS minimal medium containing high nitrogen (10 mM NH4Cl) and low nitrogen (3 mM NH4Cl) and extracted the total RNA from exponential phase and stationary phase culture. We also grew E. coli MG1655 ∆glnG StrR CamR in MOPS minimal medium containing low nitrogen (3 mM NH4Cl) and extracted the total RNA from exponential phase culture. The extracted RNA samples were sequenced, and differential expression analysis was done to determine the genes related to uptake and degradation of nitrogenous compounds that are important for E. coli colonization. Our results indication nitrogen is not limiting for E. coli in the intestine and genes related to uptake and degradation of several aminoacids, di- and tripeptides, aminosugars, ethanolamine, urea among others are upregualated in the mouse intestine as compared to invitro culture grown in high nitrogen conditions.

2024-01-29 | GSE241658 | GEO

Gene expression profiles in the bovine corpus luteum (CL) of the estrous cycle and pregnancy: Possible roles of chemokines in regulating CL function during pregnancy

Project description:To determine functional differences between the corpus luteum (CL) of the estrous cycle and pregnancy in cows, gene expression profiles between the CL of the estrous cycle and pregnancy were investigated. A 15 K bovine oligo DNA microarray detected 138, 265 and 455 differentially expressed genes (>2-fold; P<0.05) in the bovine CL of 20-25, 40-45, and 150-160 days of pregnancy compared with 10-12 days of the estrous cycle. The different gene expression profiles may contribute to functional differences between the CL of pregnancy and the CL of the estrous cycle in cows. Chemokines including eotaxin and lymphotactin may regulate CL function during pregnancy. Bovine ovaries containing corpora lutea (CLs) were obtained from Japanese-Black cows in the institute ranch within 10-30 min of exsanguination. Tissue samples were collected from cows on 10-12 days of the estrous cycle (cyclic), 20-25, 40-45, and 150-160 days of the gestation (n=4 animals/stage). The day of artificial insemination was designated as day 1.

2014-08-10 | E-GEOD-59770 | biostudies-arrayexpress

A proteomics analysis of fnr regulation of anaerobic metabolism of food dye and drug metabolism by Escherichia coli

Project description:Proteomic data from E. coli grown with or without the azo dye, Red Dye number 40. WT and delta fnr E. coli were cultured in Luria Broth media and cultures were inoculated with 250 uM Red No. 40 at either mid-exponential or stationary growth phases. Vehicle control samples were also analyzed at mid-exponential and stationary growth phases.

2023-07-06 | MSV000092365 | MassIVE