Project description:Old C57BL/6 mice cannot mount an effective innate immune response We used mouse microarrays to detail the molecular profile of the events that occur following infection
Project description:The elderly have increased morbidity and mortality following sepsis; however, the cause(s) remains unclear. We hypothesized that these poor outcomes are due in part to defects in innate immunity, rather than to an exaggerated early inflammatory response. Young (6-12 wk) or aged (20-24 mo) mice underwent polymicrobial sepsis, and subsequently, the aged mice had increased mortality and defective peritoneal bacterial clearance compared with young mice. No differences were found in the magnitude of the plasma cytokine responses. Although septic aged mice displayed equivalent or increased numbers of circulating, splenic, and bone marrow myeloid cells, some of these cells exhibited decreased phagocytosis, reactive oxygen species production, and chemotaxis. Blood leukocyte gene expression was less altered in aged versus young mice 1 d after sepsis. Aged mice had a relative inability to upregulate gene expression of pathways related to neutrophil-mediated protective immunity, chemokine/chemokine receptor binding, and responses to exogenous molecules. Expression of most MHC genes remained more downregulated in aged mice at day 3. Despite their increased myeloid response to sepsis, the increased susceptibility of aged mice to sepsis appears not to be due to an exaggerated inflammatory response, but rather, a failure to mount an effective innate immune response.
Project description:Analysis of plasma proteomes from 8 week old C57BL/6J WT or TCRα-/- (Tcratm1Phi) either naïve or infected by intravenous injection of 5x105 yeast (clinical isolate SC5314), and collected 72 hrs post-infection
Project description:Platelets were isolated from standard-housed and exercising (4 days and 28 days) 18-month-old C57BL/6J mice and mass spectrometry performed. This analysis revealed differential proteomic signatures between platelets from exercsising and standard-housed mice.
Project description:Microbeads (experimental glaucoma) or saline solution (controls) were injected into right anterior chamber of young adult mice to induce high intraocular pressure in glaucoma group. In addition, 13 week old and 40 week old naïve C57bl/6 mice were also examined.
Project description:To investigate the effect of aging on liver endothelial cells, we performed RNA sequencing of liver endothelial cells isolated from young (10-week-old) and aged (127-136 week-old) C57BL/6J mice.