Proteomics

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Verfication of immunogenic nature by microarray screening


ABSTRACT: Screening of 14 novel proteins derived from Klebsiella pneumoniae MGH 78578 identified prior via screening of cDNA libraries. The full-length proteins were attached using a specific HaloTag to their corresponding ligand surface, HaloLink. Screening was performed using two different polyclonal antibodies to Klebsiella pneumoniae (Acris AP00792PU-N and Abcam ab20947) and detection achieved by Goat polyclonal to rabbit IgG conjugated with Chromeo-546 (Abcam ab60317). In order to assess their potential immungenic nature and rank the proteins investigated, comparative analysis using already described antigens from K. pneumoniae were used in the assay.

ORGANISM(S): Klebsiella pneumoniae

PROVIDER: GSE52538 | GEO | 2014/02/02

SECONDARY ACCESSION(S): PRJNA229172

REPOSITORIES: GEO

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