Project description:In order to identify gene-expression patterns in mesenchymal stem cells associated with different birth weights and intrauterine growth parameters, we conducted comparative microarray gene expression experiments prior and post acute insulin stimulation Samples were collected from umbilical cord derived mesenchymal stem cells (UC-MSC). 4 samples belong to SGA (Small for Gestational Age) and 4 samples belong to control group

2014-03-31 | E-GEOD-52841 | biostudies-arrayexpress

Molecular pathways reflecting poor intrauterine growth are imprinted in WhartonM-bM-^@M-^Ys jelly derived Mesenchymal Stem Cells [Set2]

Project description:In order to identify gene-expression patterns in mesenchymal stem cells associated with different birth weights and intrauterine growth parameters, we conducted comparative microarray gene expression experiments prior and post acute insulin stimulation Mesenchymal stem cells (MSC) were extracted from the Wharton's jelly of the umbilical cord of normal and SGA babies. The MSCs were expended in culture. The MSCs were subjected to acute insulin treatment for various time points. The RNA of these cells were collected for gene-expression study.

2014-03-31 | E-GEOD-52842 | biostudies-arrayexpress

Molecular pathways reflecting poor intrauterine growth are imprinted in WhartonM-bM-^@M-^Ys jelly derived Mesenchymal Stem Cells [set4]

Project description:In order to identify gene-expression patterns in mesenchymal stem cells associated with different birth weights and intrauterine growth parameters, we conducted comparative microarray gene expression experiments prior and post acute insulin stimulation Mesenchymal stem cells (MSC) were extracted from the Wharton's jelly of the umbilical cord of normal and SGA babies. The MSCs were expended in culture. The MSCs were subjected to acute insulin treatment for various time points. The RNA of these cells were collected for gene-expression study.

2014-03-31 | E-GEOD-52844 | biostudies-arrayexpress

Molecular pathways reflecting poor intrauterine growth are imprinted in WhartonM-bM-^@M-^Ys jelly derived Mesenchymal Stem Cells [set3]

Project description:In order to identify gene-expression patterns in mesenchymal stem cells associated with different birth weights and intrauterine growth parameters, we conducted comparative microarray gene expression experiments prior and post acute insulin stimulation Mesenchymal stem cells (MSC) were extracted from the Wharton's jelly of the umbilical cord of normal and SGA babies. The MSCs were expended in culture. The MSCs were subjected to acute insulin treatment for various time points. The RNA of these cells were collected for gene-expression study.

2014-03-31 | E-GEOD-52843 | biostudies-arrayexpress

Culturing on Wharton's jelly extract delays mesenchymal stem cell senescence through p53 and p16INK4a/pRb pathways.

Project description:Mesenchymal stem cells (MSCs) hold great therapeutic potential. However, MSCs undergo replication senescence during the in vitro expansion process. Wharton's jelly from the human umbilical cord harbors a large number of MSCs. In this study, we hypothesized that Wharton's jelly would be beneficial for in vitro expansion of MSCs. Wharton's jelly extract (WJEs), which is mainly composed of extracellular matrix and cytokines, was prepared as coating substrate. Human MSCs were isolated and cultured on WJE-coated plates. Although the proliferation capacity of cells was not augmented by WJE in early phase culture, adynamic growth in late-phase culture was clearly reduced, suggesting that the replicative senescence of MSCs was efficiently slowed by WJE. This was confirmed by ?-galactosidase staining and telomere length measurements of MSCs in late-phase culture. In addition, the decreased differentiation ability of MSCs after long-term culture was largely ameliorated by WJE. Reactive oxygen species (ROS), p53, and p16INK4a/pRb expression increased with passaging. Analysis at the molecular level revealed that WJE-based culture efficiently suppressed the enhancement of intracellular ROS, p53, and p16INK4a/pRb in MSCs. These data demonstrated that WJE provided an ideal microenvironment for MSCs culture expansion in vitro preserved MSC properties by delaying MSCs senescence, and allowed large numbers of MSCs to be obtained for basic research and clinical therapies.

| S-EPMC3596399 | biostudies-literature

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