Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Medicago truncatula seed development at 14/11°C 16 light/dark

ABSTRACT: Transcriptional profiling of seeds of Medicago truncatula during maturation. To identify genes that are regulated during seed maturation in the model legume Medicago truncatula, plants at flowering stage were grown at controlled temperature of 14/11°C, 16h light/dark. Seeds were then collected at different stages of development. Using the Medicago NimbleGen chip, a transcriptomic analysis was performed to follow the differential expression of genes during seed maturation.

ORGANISM(S): Medicago truncatula

PROVIDER: GSE53002 | GEO | 2013/12/18

SECONDARY ACCESSION(S): PRJNA231950

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Medicago truncatula seed development at 21-19°C

2013-07-31 | GSE49350 | GEO

Medicago truncatula seed development under greenhouse conditions

2019-09-11 | GSE137174 | GEO

Medicago truncatula seed development at under greenhouse conditions

2013-12-05 | GSE52833 | GEO

Medicago truncatula seed development at 26/24°C 16 h light/dark

2013-12-05 | GSE52832 | GEO

Medicago truncatula seed development at 20/18°C 16 h light/dark in osmotic stress conditions: -0.1 Mpa

2013-12-05 | GSE53003 | GEO

Medicago truncatula seed development at 14/11Â°C 16 light/dark

Project description:Transcriptional profiling of seeds of Medicago truncatula during maturation. To identify genes that are regulated during seed maturation in the model legume Medicago truncatula, plants at flowering stage were grown at controlled temperature of 14/11Â°C, 16h light/dark. Seeds were then collected at different stages of development. Using the Medicago NimbleGen chip, a transcriptomic analysis was performed to follow the differential expression of genes during seed maturation. Seeds of Medicago truncatula grown at 14/11Â°C, 16h light/dark were collected at different developmental stages, 10 developmental stages were analysed. Two replicates from each developmental stage were used for dye switch, each time the control was considered as the earlier developmental stage vs the treatment, corresponding to the later developmental stage. For each repetition 5 hybridisation were made: 22DAP vs 28DAP, 34DAP vs 40DAP, 46DAP vs 52DAP, 58DAP vs 65DAP,Abs vs DS. For each biological replicates, RNA was extracted from 50 seeds collected from 5 different plants, grown in the same conditions. One replicate per array.

2013-12-18 | E-GEOD-53002 | biostudies-arrayexpress

Medicago truncatula seed development at 21-19Â°C

Project description:Transcriptional profiling of seeds of Medicago truncatula during maturation. To identify genes that are regulated during seed maturation in the model legume Medicago truncatula, plants at flowering stage were grown at controlled temperature of 21-19Â°C, 16h light. Seeds were then collected at different stages of development. Using the Medicago NimbleGen chip, a transcriptomic analysis was performed to follow the differential expression of genes during seed maturation. Seeds of Medicago truncatula grown at 21-19Â°C were collected at different developmental stages, 15 developmental stages were analysed. Two replicates from each developmental stage were used for dye switch, each time the control was considered as the earlier developmental stage vs the treatment, corresponding to the later developmental stage. For each repetition 8 hybridisation were made: 8DAP vs 11DAP, 14DAP vs 17DAP, 20DAP vs 23DAP, 26DAP vs 29DAP,32DAP vs 35DAP, 38DAP vs 41DAP, 44DAP vs Abs, DS (Rep1 and Rep2) vs 8DAP (Rep3 and Rep4). For each biological replicates, RNA was extracted from 50 seeds collected from 5 different plants, grown in the same conditions. One replicate per array.

2013-07-31 | E-GEOD-49350 | biostudies-arrayexpress

Medicago truncatula seed development at under greenhouse conditions

Project description:Transcriptional profiling of seeds of Medicago truncatula during maturation. To identify genes that are regulated during seed maturation in the model legume Medicago truncatula, plants at flowering stage were grown at variable light and temperature conditions under greenhouse environment (period March-June). Seeds were then collected at different stages of development. Using the Medicago NimbleGen chip, a transcriptomic analysis was performed to follow the differential expression of genes during seed maturation. Seeds of Medicago truncatula were collected at different developmental stages, 9 developmental stages were analysed. Two replicates from each developmental stage were used for dye switch, each time the control was considered as the earlier developmental stage vs the treatment corresponding to the later developmental stage. For each repetition 5 hybridisation were made: 16DAP vs 20DAP, 24DAP vs 28DAP, 32DAP vs 36DAP, 40DAP vs Abs, DS (Rep1 and Rep2) vs 16DAP (Rep3 and Rep4). For each biological replicates, RNA was extracted from 50 seeds collected from 5 different plants, grown in the same conditions. One replicate per array.

2013-12-05 | E-GEOD-52833 | biostudies-arrayexpress

Medicago truncatula seed development at 26/24Â°C 16 h light/dark

Project description:Transcriptional profiling of seeds of Medicago truncatula during maturation. To identify genes that are regulated during seed maturation in the model legume Medicago truncatula, plants at flowering stage were grown at controlled temperature of 26/24Â°C 16 h light/dark. Seeds were then collected at different stages of development. Using the Medicago NimbleGen chip, a transcriptomic analysis was performed to follow the differential expression of genes during seed maturation. Seeds of Medicago truncatula grown at 26/24Â°C 16 h light/dark were collected at different developmental stages, 8 developmental stages were analysed. Two replicates from each developmental stage were used for dye switch, each time the control was considered as the earlier developmental stage vs the treatment corresponding to the later developmental stage. For each repetition 4 hybridisation were made: 7DAP vs 9DAP, 11DAP vs 14DAP, 17DAP vs 20DAP, Abs vs DS. For each biological replicates, RNA was extracted from 50 seeds collected from 5 different plants, grown in the same conditions. One replicate per array.

2013-12-05 | E-GEOD-52832 | biostudies-arrayexpress

Medicago truncatula late seed maturation

Project description:Seven developmental stages of Medicago truncatula A17 during seed maturation (from 24 DAP to dry seeds)

2013-07-23 | E-MEXP-3719 | biostudies-arrayexpress