Other

Dataset Information

0

Replication timing of control and overexpressed PolQ fibroblast cells


ABSTRACT: The human A-family DNA polymerase θ (Pol q) is a large, multidomain enzyme whose physiological function is still unclear despite its in vitro translesion synthesis capacity in front of DNA damage and its involvement in some features of DNA repair after external stress. Here we present evidence that Pol q holds a novel role in the absence of external stress as a critical determinant of the replication timing program in human cells. Pol q binds to chromatin at early G1 and is required for proper formation of pre-replicative complexe and replication origin activation. Pol q-depleted cells show modified spatial organization of chromatin-loop structures at replication factories. Genome-wide analysis of replication timing shows delayed replication of a part of early replicating domains and advanced replication of a part of late replicating domains following Pol q depletion. Our results identify Pol q as one of the first critical human factors discovered in the replication timing programme.

ORGANISM(S): Homo sapiens

PROVIDER: GSE53070 | GEO | 2014/10/28

SECONDARY ACCESSION(S): PRJNA230790

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2014-06-19 | E-GEOD-49693 | biostudies-arrayexpress
2014-10-28 | E-GEOD-53070 | biostudies-arrayexpress
2014-06-19 | GSE49693 | GEO
2019-01-23 | GSE98730 | GEO
2019-01-23 | GSE124274 | GEO
2019-01-23 | GSE98714 | GEO
2018-10-19 | GSE115847 | GEO
2021-09-21 | GSE159664 | GEO
2021-09-21 | GSE159663 | GEO
2021-09-21 | GSE158010 | GEO