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Transcriptome (Affymetrix arrays) and Methylome of HepG2 cells uninfected (Cont) or infected with the pathogen Listeria monocytogenes (Inf)

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Note that transcriptomes are performed with Affymetrix arrays and samples noted "S72h" have been sorted by FACS.

ORGANISM(S): Homo sapiens

PROVIDER: GSE53367 | GEO | 2021/12/31

REPOSITORIES: GEO

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Transcriptome (Affymetrix arrays) of HepG2 cells uninfected (Cont) or infected with the pathogen Listeria monocytogenes (Inf) for 24 or 72 hours.

Project description:Comparisons of gene expression profiles of human hepatocytes (HepG2) infected or not by the bacterium Listeria monocytogenes (strain EGD-e expressing GFP) for 24 or 72 hours and use of Affymetrix microarrays. Samples noted S72h have been sorted by FACS.

2021-12-31 | GSE53285 | GEO

Methylome of of HepG2 cells uninfected ("Cont") or infected with the pathogen Listeria monocytogenes ("Inf") by Methyl-binding domain-sequencing (MBD-Seq/MethylCap-Seq)

Project description:We report the partial methylome (CG-rich regions) of human hepatocytes (HepG2) infected or not by the bacterium Listeria monocytogenes

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Listeria monocytogenes EGD and EGD-e

Project description:Transcriptional profile after growth in MM (minimal medium (Premaratne et al. 2001) supplemented with 50 mM Glucose. Note: strain "EGD" has not been deposited at ATCC/NCTC. It is a derivative of the original Murray strain (Murray et al. 1926), obtained from S.H.E. Kaufmann. Strain "EGD-e" has been obtained from H. Hahn (Berlin) via T. Chakraborty (Gießen) and is deposited under ATTC BAA-679 and in the Pasteur culture collection. Keywords: strain comparison

2008-12-01 | GSE12146 | GEO

Transcriptome (RNA-seq) of Huh7 cells non-infected (NI) or infected (Inf.) with the pathogen Listeria monocytogenes (strain EGD-e)

Project description:Comparisons of gene expression profiles of human hepatocytes (Huh7) infected or not by the bacterium Listeria monocytogenes (strain EGD-e) for 72 hours and analysed by RNA-seq

2021-09-26 | GSE184729 | GEO

Transcriptome (RNA-seq) of HepG2 cells non-infected (NI) or infected (Inf.) with the pathogen Listeria monocytogenes (strain EGD-e)

Project description:Comparisons of gene expression profiles of human hepatocytes (HepG2) infected or not by the bacterium Listeria monocytogenes (strain EGD-e) for 72 hours and analysed by RNA-seq

2021-09-25 | GSE184697 | GEO

Transcriptome (RNA-seq) of primary mouse hepatocytes (PMH) cells non-infected (NI) or infected (Inf.) with the pathogen Listeria monocytogenes (strain 10403S)

Project description:Comparisons of gene expression profiles PMH infected or not by the bacterium Listeria monocytogenes (strain 10403S) for 72 hours and analysed by RNA-seq

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Listeria monocytogenes EGD and EGD-e

2008-11-30 | E-GEOD-12146 | biostudies-arrayexpress

The virulence gene cluster of Listeria monocytogenes is also present in Listeria ivanovii, an animal pathogen, and Listeria seeligeri, a nonpathogenic species.

Project description:Most known Listeria monocytogenes virulence genes cluster within a 9.6-kb chromosomal region. This region is flanked on one end by two uncharacterized open reading frames (ORF A and ORF B) and ldh, an ORF presumably encoding the L. monocytogenes lactate dehydrogenase (J.-A. Vazquez-Boland, C. Kocks, S. Dramsi, H. Ohayon, C. Geoffroy, J. Mengaud, and P. Cossart, Infect. Immun. 60:219-230, 1992). We report here that the other end is flanked by prs, and ORF homologous to phosphoribosyl PPi synthetase genes. ORF B and prs were detected in all Listeria species and thus delimit the virulence region. This virulence gene cluster was detected exclusively in hemolytic Listeria species, Listeria ivanovii, an animal pathogen, and Listeria seeligeri, a nonpathogenic species.

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Listeria monocytogenes meningitis.

Project description:Tumbling motility is one of the useful characteristics of Listeria monocytogenes. This can be helpful to identify the causative pathogen along with Gram staining before the confirmatory microbiological examination.

| S-EPMC5986172 | biostudies-other

Evidence of Biocontrol Activity of Bioinoculants Against a Human Pathogen, Listeria monocytogenes.

Project description:Due to rhizodeposits and various microbial interactions, the rhizosphere is an extremely dynamic system, which provides a conductive niche not only for bacteria beneficial to plants but also for those that might pose a potential threat to humans. The importance of bioinoculants as biocontrol agents to combat phytopathogens has been widely recognized. However, little information exists with respect to their role in inhibiting human pathogens in the rhizosphere. The present study is an attempt to understand the impact of an established bacterial consortium, Azotobacter chroococcum, Bacillus megaterium, and Pseudomonas fluorescens, on the survivability of Listeria monocytogenes in the rhizosphere of Cajanus cajan and Festuca arundinacea. An experiment conducted in Hoagland's medium in the presence of C. cajan demonstrated that the presence of bioinoculants impaired growth of L. monocytogenes compared to that observed in their absence. On the other hand, in the presence of F. arundinacea, no significant differences were observed in the population dynamics of L. monocytogenes in the presence or absence of the bioinoculants. Agar plate assay through cross streak method revealed the inhibition of L. monocytogenes by bioinoculants. Potential bioactive compounds were identified by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). These results suggest that agricultural amendments can act as protective agents against human pathogens while enforcing plant growth promotion.

| S-EPMC7078112 | biostudies-literature

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