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Detection of juvenile hormone-like function of diofenolan in Daphnia magna using in vivo short-term screening assay, TG211 Annex 7 reproduction test and microarray analysis

ABSTRACT: Juvenile hormone (JH) and JH agonists have been reported to induce male offspring production in various daphnids including Daphnia magna. We have recently established a short-term in vivo screening method to detect chemicals having JH activity, induction of male offspring, using adult D. magna. Diofenolan has been developed to be a JH agonist in insects, but no male induction in daphniids has been studied. In this study, we found the male offspring induction by diofenolan with a short-term in vivo screening method, and the most potent male offspring production activity among JH analogs studied using the OECD TG211 Annex 7. Transcriptome analysis of diofenolan-exposed D. magna showed up-regulation of JH-responsive genes. These results clearly demonstrated that diofenolan has JH activities in D. magna using the short-term in vivo screening assay, OECD TG211 Annex 7 and microarray analysis.

ORGANISM(S): Daphnia magna

PROVIDER: GSE53570 | GEO | 2014/12/01

SECONDARY ACCESSION(S): PRJNA232398

REPOSITORIES: GEO

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