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Expression data from Arabidopsis C3H14/15 overexpressors, c3h14c3h15 double mutant vs wild type

ABSTRACT: In plants, CCCH zinc finger proteins involved in secondary wall formation and anther development are poorly understood. We have functionally identified two homologous genes C3H14 and C3H15 and found that the two genes differentially regulate secondary wall formation and anther development. C3H14 contributes more to secondary wall thickening, whereas, C3H15 is more important for anther development. We performed microarray analyses on C3H14/15 overexpressors and c3h14c3h15 double mutant to to ientify differentially-expressed genes involved in the two developmental processes.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE53580 | GEO | 2015/04/07

SECONDARY ACCESSION(S): PRJNA232445

REPOSITORIES: GEO

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Expression data from Arabidopsis C3H14/15 overexpressors, c3h14c3h15 double mutant vs wild type

Project description:In plants, CCCH zinc finger proteins involved in secondary wall formation and anther development are poorly understood. We have functionally identified two homologous genes C3H14 and C3H15 and found that the two genes differentially regulate secondary wall formation and anther development. C3H14 contributes more to secondary wall thickening, whereas, C3H15 is more important for anther development. We performed microarray analyses on C3H14/15 overexpressors and c3h14c3h15 double mutant to to ientify differentially-expressed genes involved in the two developmental processes. Our microarray data show that C3H14 and C3H15 regulate the expression of a number of genes involved in various biological activities, particularly those associated with secondary wall metabolism and pollen formation.

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NST1 over-expression vs wild type

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Histone H3K4 methyltransferase ATX1 positively regulates secondary wall biosynthesis in interfascicular fibers in Arabidopsis

Project description:Secondary wall thickening in the sclerenchyma cells is strictly controlled by a complex network of transcription factors in vascular plant. However, little is known about the epigenetic mechanism regulating secondary cell wall biosynthesis. Genome-wide analysis revealed that the up-regulation of genes involved in secondary wall formation during stem development is largely coordinated by increasing level of H3K4 tri-methylation. In this study, we identified that ARABIDOPSIS HOMOLOG of TRITHORAX1 (ATX1), a H3K4-histone methyltransferase, positively regulates secondary wall deposition mainly through activating the expression of secondary wall NAC master switch genes, SECONDARY WALL-ASSOCIATED NAC DOMAIN PROTEIN1 (SND1) and NAC SECONDARY WALL THICKENING PROMOTING FACTOR1 (NST1).

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AtC3H14, a direct target of MYB46, suppress cell elongation and involved in the secondary wall formation.

Project description:Recently, AtC3H14, a CCCH-type zinc finger protein, was identified as one of the direct targets of MYB46, which is known as a master regulator of secondary wall biosynthesis. AtC3H14 and their homologs (i.e., AtC3H15 and PtrC3H14-1 from Arabidopsis and poplar, respectively) are predominantly expressed in the secondary wall forming tissues. Transgenic Arabidopsis plants overexpressing AtC3H14 (i.e., 35S::AtC3H14 plants) produced dwarfing phenotypes. 35S::AtC3H14 plants developed phloem fibers earlier than wild-type and this phenotype was more pronounced in the roots. Interestingly, ectopic secondary wall thickenings were found in both stems and roots. These phenotypic consequences are successively reproduced from the 35S::AtC3H15 and 35S::PtrC3H14-1 plants. Whole transcriptome GeneChip analysis identified that the ‘cell wall’ and ‘extracellular’-related genes are extremely over-represented in the stem tissues of 35S::AtC3H14 plants. These results suggest that AtC3H14 may act as a negative regulator of cell elongation with modification of cell wall reassembly and be involved in the secondary wall formation in Arabidopsis.

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