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NELAVL HITS-CLIP and RNA-seq in Alzheimer's Disease patients and IMR-32 neuroblastoma cells

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE53699 | GEO | 2016/02/17

SECONDARY ACCESSION(S): PRJNA232667

REPOSITORIES: GEO

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nELAVL HITS-CLIP and RNA-seq in Alzheimer's Disease patients and IMR-32 neuroblastoma cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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nELAVL HITS-CLIP in IMR-32 neuroblastoma cells

Project description:Non-stressed and UV-stressed IMR-32 cells were subjected to HITS-CLIP to monitor nELAVL binding changes during AD progression

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nELAVL HITS-CLIP in IMR-32 neuroblastoma cells

Project description:Non-stressed and UV-stressed IMR-32 cells were subjected to HITS-CLIP to monitor nELAVL binding changes during AD progression Nonstressed and stressed IMR-32 cells were UV-irradiated and subjected to nELAVL HITS-CLIP (detailed desription in accompanying paper)

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nELAVL HITS-CLIP in Alzheimer's Disease patients

Project description:Human brain samples from control and advanced Alzheimer's Diseased subjects were subjected to HITS-CLIP to monitor nELAVL binding changes during AD progression Human brain samples were obtained from the Mount Sinai Brain Bank; samples were UV-irradiated and subjected to nELAVL HITS-CLIP (detailed desription in accompanying paper)

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nELAVL HITS-CLIP in Alzheimer's Disease patients

Project description:Human brain samples from control and advanced Alzheimer's Diseased subjects were subjected to HITS-CLIP to monitor nELAVL binding changes during AD progression

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RNAseq in IMR-32 neuroblastoma cells

Project description:IMR-32 cells were subjected to lentiviral YRNA infection or nELAVL RNAi and/or UV stress followed by RNAseq analysis to monitor RNA level changes

2016-02-17 | GSE53698 | GEO

RNAseq in IMR-32 neuroblastoma cells

Project description:IMR-32 cells were subjected to lentiviral YRNA infection or nELAVL RNAi and/or UV stress followed by RNAseq analysis to monitor RNA level changes RNA from IMR-32 cells was Trizol extracted, Ribominus selected and submitted for high-throughput sequencing.

2016-02-17 | E-GEOD-53698 | biostudies-arrayexpress

HITS-CLIP and RNA-Seq of bovine retina

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-09-10 | E-GEOD-59912 | biostudies-arrayexpress

Inhibition of N-myc expression sensitizes human neuroblastoma IMR-32 cells expressing caspase-8 to TRAIL.

Project description:ObjectivesThis study aims to explore the roles of N-myc and caspase-8 in TRAIL-resistant IMR-32 cells which exhibit MYCN oncogene amplification and lack caspase-8 expression.Materials and methodsWe established N-myc-downregulated IMR-32 cells using shRNA lentiviral particles targeting N-myc and examined the effect the N-myc inhibition on TRAIL susceptibility in human neuroblastoma IMR-32 cells expressing caspase-8.ResultsCisplatin treatment in IMR-32 cells increased the expression of death receptor 5 (DR5; TRAIL-R2), but not other receptors, via downregulation of NF-?B activity. However, the cisplatin-mediated increase in DR5 failed to induce cell death following TRAIL treatment. Furthermore, interferon (IFN)-? pretreatment increased caspase-8 expression in IMR-32 cells, but cisplatin failed to trigger TRAIL cytotoxicity. We downregulated N-myc expression in IMR-32 cells using N-myc-targeting shRNA. These cells showed decreased growth rate and Bcl-2 expression accompanied by a mild collapse in the mitochondrial membrane potential as compared with those treated with scrambled shRNA. TRAIL treatment in N-myc-negative cells expressing caspase-8 following IFN-? treatment significantly triggered apoptotic cell death. Concurrent treatment with cisplatin enhanced TRAIL-mediated cytotoxicity, which was abrogated by an additional pretreatment with DR5:Fc chimera protein.ConclusionsN-myc and caspase-8 expressions are involved in TRAIL susceptibility in IMR-32 cells, and the combination of treatment with cisplatin and TRAIL may serve as a promising strategy for the development of therapeutics against neuroblastoma that is controlled by N-myc and caspase-8 expression.

| S-EPMC6536445 | biostudies-literature

HITS-CLIP and RNA-Seq of bovine retina

Project description:This SuperSeries is composed of the SubSeries listed below.

2014-09-10 | GSE59912 | GEO