Genomics

Dataset Information

0

Genome-wide analysis reveals dynamic changes in microRNAs expression during vascular cambium development in Chinese fir


ABSTRACT: MicroRNAs (miRNAs) are small noncoding regulatory RNAs that play key roles in the process of plant development. To date, extensive studies of miRNAs have been performed in a few model plants, but few efforts have focused on small RNAs in conifers because of the lack of reference sequences for these enormous genomes. In this study, Solexa sequencing of three small RNA libraries obtained from dormant, reactivating, and active vascular cambium in Chinese fir samples identified 76 known miRNAs from 27 miRNA families and 18 new potential miRNAs, of which 15 novel miRNA precursors were validated by RT-PCR and sequencing. More than half of these novel miRNAs displayed stage-specific expression patterns in the vascular cambium. Furthermore, analyzing the 103 miRNAs and their predicted targets indicated that 30% of miRNAs appeared to negatively regulate their targets, of which 4 target genes involved in the regulation of cambial cell division were validated via RNA ligase-mediated rapid amplification of 5’ cDNA ends (RLM 5’-RACE). Interestingly, miRNA156 and miRNA172 may regulate the phase transition in vascular cambium from dormancy to active growth. These results provide new insights into the important regulatory functions of miRNAs in vascular cambium development and wood formation in conifers.

ORGANISM(S): Cunninghamia lanceolata

PROVIDER: GSE53933 | GEO | 2015/04/07

SECONDARY ACCESSION(S): PRJNA233997

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2015-04-07 | E-GEOD-53933 | biostudies-arrayexpress
2021-01-17 | GSE109323 | GEO
2019-03-31 | GSE125244 | GEO
2016-07-26 | PXD003504 | Pride
2015-07-21 | GSE71094 | GEO
2024-04-24 | GSE264403 | GEO
2010-12-31 | E-GEOD-22947 | biostudies-arrayexpress
2010-12-31 | GSE22947 | GEO
2016-11-26 | GSE53034 | GEO
2009-12-22 | GSE18675 | GEO