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Expression profiling of VCaP prostate cancer cells with ERG knockdown or WP1130 treatment

ABSTRACT: Microarray studies was performed to analyze gene expression profiling in VCaP cells after ERG was silenced by two different siRNAs or VCaP cells were treated by WP1130.

ORGANISM(S): Homo sapiens

PROVIDER: GSE53994 | GEO | 2014/03/03

SECONDARY ACCESSION(S): PRJNA234296

REPOSITORIES: GEO

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Expression profiling of VCaP prostate cancer cells with ERG knockdown or WP1130 treatment

Project description:Microarray studies was performed to analyze gene expression profiling in VCaP cells after ERG was silenced by two different siRNAs or VCaP cells were treated by WP1130. RNA samples, containing 3 replicates for each sample, were extracted from VCaP cells at 72 hours siERG post-transfection or 24 hours WP1130 treatment. After biotin labeling, samples were hybrided on the Illumina Human HT12v4.0 Expression Beadchip

2014-03-03 | E-GEOD-53994 | biostudies-arrayexpress

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The effect of ERG overexpression and ERG knockdown by shRNA in RWPE1 and VCAP cells, respectively

Project description:The goal of this project was to analyze the global gene expression profiles of RWPE1 and VCAP cells following transfection of GFP, GFP-ERG at 48 and 72hrs time points and stable ERG shRNA, scramble shRNA, respectively. RWPE1 cells were transfected with GFP or GFP-ERG. VCAP cells were transfected with ERG lenti-shRNA or scramble shRNA. Transfections were performed in duplicate. Total cellular RNA was isolated with Trizol and quality analysed by the bioanalyser kit.

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Expression profiling of human prostate VCaP and LNCaP cancer cells after silencing ERG or ETV1, respectively

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2013-03-01 | E-GEOD-39354 | biostudies-arrayexpress

Testing gene expression changes in VCaP upon depletion of the mutated ETS transcription factor ERG

Project description:VCaP cells expressing inducible shRNAs for either ERG or a non-targeting control were treated with Doxycycline for 1, 3, 7 and 10 days prior to collection This experiment is designed to see which genes and pathways are modulated by ERG knockdown VCaP cells stably expressing a Doxycycline (dox)-inducible control nontargeting shRNA (Pak4) or an ERG shRNA (2217) were exposed to 100ng/ml Dox for the noted days.

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Testing gene expression changes in VCaP upon depletion of the mutated ETS transcription factor ERG

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Project description:The aberrant activation of the ERG oncogenic pathway due to TMPRSS2-ERG gene fusions is the major driver of prostate cancer initiation and progression. We identified the alpha1 and beta1 subunits of soluble guanylyl cyclase (GUCY1A1, GUCY1B1) as major ERG-regulated genes in prostate cancer cells. Soluble guanylyl cyclase (sGC) is the major mediator of nitric oxide signaling in cells that, upon nitric oxide binding, catalyzes the synthesis of cGMP and subsequently activates PKG. We showed in ERG-positive PCa cells (VCaP) that cGMP synthesis was significantly elevated by ERG, leading to increased PKG activity and cell proliferation. To further understand the functions of sGC-cGMP pathway in prostate cancer cells, we performed RNA-seq analyses in VCaP cells to identify genes that are regulated by sGC.

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