Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression profiles of field-grown wheat expressing the Pm3 allele and null segregants

ABSTRACT: It has been demonstrated that wheat overexpressing Pm3b, an allele of the R gene Pm3, has enhanced resistance against powdery mildew under field conditions. A gene expression profile study using GeneChip® Wheat Genome Array was performed to obtain insights into the mode of action of Pm3b and to elucidate the molecular basis of pleiotropic effects observed in three out of four independent transgenic events tested under field conditions.

ORGANISM(S): Triticum aestivum

PROVIDER: GSE54260 | GEO | 2014/05/01

SECONDARY ACCESSION(S): PRJNA236053

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Expression profiles of field-grown wheat expressing the Pm3 allele and null segregants

Project description:It has been demonstrated that wheat overexpressing Pm3b, an allele of the R gene Pm3, has enhanced resistance against powdery mildew under field conditions. A gene expression profile study using GeneChipÂ® Wheat Genome Array was performed to obtain insights into the mode of action of Pm3b and to elucidate the molecular basis of pleiotropic effects observed in three out of four independent transgenic events tested under field conditions. 24 samples were analyzed. Three biological replicates of each sample were included. Two independent transformation events and respective null segregants as control treated with fungicide or artificially inoculated with powdery mildew.

2014-05-01 | E-GEOD-54260 | biostudies-arrayexpress

Expression data in wheat (T. aestivum L.) near isogenic lines in response to powdery mildew infection

2011-02-15 | GSE27320 | GEO

Expression data in wheat (T. aestivum L.) near isogenic lines in response to powdery mildew infection

Project description:We used two wheat genotypes, the susceptible wheat cultivar ‘8866 ’(S) and its near isogenic line with single powdery mildew resistance gene ‘pm30’ (R), to investigate gene expression changes in response to powdery mildew infection by using Wheat Genome Array wheat young leveas of near isogenic lines before or 12 hours after powdery mildew infection were selected for RNA extraction and hybridization on Affymetrix microarrays.The leaf samples were harvested from three independent biological replicates, and the leaves without inoculation were regarded as control.

2011-02-15 | E-GEOD-27320 | biostudies-arrayexpress

Expression data from wheat cultivars

2014-01-30 | GSE43930 | GEO

Expression data from wheat cultivars

Project description:To identify genes involved in susceptibility, genechip hybridization experiments were performed in order to examine genes differentially expressed upon inoculation of resistant and susceptible wheat cultivars with powdery mildew. Some genes were identified which were just expressed in the susceptible host both after mock-inoculation and pathogen infection. Also, a total of 2693 transcripts were differentially expressed (fold change≥2) in Yumai 13 in response to powdery mildew as compared to itself, comprising 1464 and 1229 up- and down-regulated genes respectively. Seven-day-old wheat seedlings of susceptible cultivar Yumai 13, two resistant cultivars HY and CYC were inoculated with powdery mildew and harvested at 0, 24, 48 hpi for RNA extraction and hybridization on Affymetrix microarrays. We sought to screen some genes which have very high expression in Yumai 13, but not in CYC and HY by pairwise comparation.

2014-01-30 | E-GEOD-43930 | biostudies-arrayexpress

Genome-wide sequencing small RNAs in response to powdery mildew and heat stress in wheat

Project description:To test whether non-coding RNAs play roles in regulating response to powdery mildew infection and heat stress in wheat, by using Solexa high-throughput sequencing and computational analysis and experimental approach we cloned the small RNAs and identified 125 putative long npcRNAs from wheat leaves infected by preponderant physiological strain Erysiphe graminis f. sp. tritici (Egt) or by heat stress treatment. Among long non-coding RNAs, some were precursors of small RNAs such as microRNAs and siRNAs, two long npcRNAs were identified as signal recognition particle (SRP) 7S RNA variants, and three were characterized as U3 snoRNAs. Wheat long npcRNAs showed tissue dependent expression patterns and were responsive to powdery mildew infection and heat stress.

2011-02-15 | GSE27327 | GEO

Genome-wide sequencing small RNAs in response to powdery mildew and heat stress in wheat

2011-02-15 | E-GEOD-27327 | biostudies-arrayexpress

Identification of candidate genes for nonhost resistance to powdery mildew in wheat

Project description:To identify the nonhost resistance genes, a time-series based global expression profiling was performed in wheat using Agilent gene expression 44K microarray array. Seven-day-old wheat plants of cv. Renan was inoculated with spore density of 50-80 conidia mm-2 of adapted (Bgt) or non-adapted (Bgh) powdery mildew pathogen, and the abaxial epidermis of inoculated primary leaves or from non-inoculated control leaves was peeled at 6, 12, 24 and 74 h after inoculation. Total RNA was extracted by using the RNeasy plant mini kit with on-Column DNase digestion (Qiagen, Hilden, Germany), and hybridized to a 44K Agilent oligonucleotide custom array of wheat.

2016-06-30 | E-MTAB-3803 | biostudies-arrayexpress

Transcription profiling of wild type and loss-of-function mutants of Mla1 and Mla6 powdery mildew resistant allele barley plants

Project description:A parallel expression profiling of wild-type and loss-of-function mutants of Mla6 and Mla1 powdery mildew resistance alleles was conducted using Barley1 GeneChip. Barley plants were inoculated with powdery mildew isolate 5874 and first leaves were harvested at 6 time points after pathogen inoculation. This experiment was conducted in split-split-plot experimental design with 3 replications.

2008-11-19 | E-TABM-142 | biostudies-arrayexpress

Transcription profiling of field grown wheat treated with 3 herbicides

Project description:Wheat plants, grown in the field, were treated with 3 herbicides commonly applied on this crop to determine potential changes in gene expression.

2005-09-01 | E-MEXP-330 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data