12plex_vitis_2011_02_24h
Ontology highlight
ABSTRACT:
ORGANISM(S): Vitis vinifera
PROVIDER: GSE54346 | GEO | 2014/09/01
SECONDARY ACCESSION(S): PRJNA236245
REPOSITORIES: GEO
Similar Datasets
Project description:12plex_vitis_2011-02 - 12plex_vitis_2011_02_24h - Biological question (15 lines max): Identification of grape genes regulated in response to resistance inducer treatments. This transcriptomic analysis should provide insight in the mode of action of the compounds under study and yield generic data relevant to grape defense reaction. - Identification of grape genes regulated in response to resistance inducer treatments. This transcriptomic analysis should provide insight in the mode of action of the compounds under study and yield generic data relevant to grape defense reaction.
2017-04-17 | GSE97700 | GEO
Project description:12plex_vitis_2011-02 - 12plex_vitis_2011_02_24h - Biological question (15 lines max): Identification of grape genes regulated in response to resistance inducer treatments. This transcriptomic analysis should provide insight in the mode of action of the compounds under study and yield generic data relevant to grape defense reaction. 17 dye-swap - normal vs disease comparison,treated vs untreated comparison
2014-09-01 | E-GEOD-54346 | biostudies-arrayexpress
Project description:12plex_vitis_2011-02 - 12plex_vitis_2011_02_48h - Biological question (15 lines max): Identification of grape genes regulated in response to resistance inducer treatments. This transcriptomic analysis should provide insight in the mode of action of the compounds under study and yield generic data relevant to grape defense reaction. - The experiment took place in the greenhouse. Six week-herbaceous grape cutting (cv Marselan) were spray-treated with 5 resistance inducers, an adjuvant (used along with some inducers) and water as control. Plants were inoculated by Plasmopara viticola (downy midew) 24 h post-treatment. We used 5 plants per treatment. Leaf samples were harvested 24 h post-treatment and 48 h post-inoculation. Three biological repetitions were done. Each hybridization experiment was performed in comparison with water.
2017-04-17 | GSE97699 | GEO
Project description:12plex_vitis_2011_02_48h - Biological question (15 lines max): Identification of grape genes regulated in response to resistance inducer treatments. This transcriptomic analysis should provide insight in the mode of action of the compounds under study and yield generic data relevant to grape defense reaction. The experiment took place in the greenhouse. Six week-herbaceous grape cutting (cv Marselan) were spray-treated with 5 resistance inducers, an adjuvant (used along with some inducers) and water as control. Plants were inoculated by Plasmopara viticola (downy midew) 24h post-treatment. We used 5 plants per treatment. Leaf samples were harvested 24h post-treatment and 48h post-inoculation. Three biological repetitions were done. Each hybridization experiment was performed in comparison with water. 18 dye-swap - normal vs disease comparison
2014-09-01 | E-GEOD-54345 | biostudies-arrayexpress
Project description:12plex_vitis_2011_02_48h - Biological question (15 lines max): Identification of grape genes regulated in response to resistance inducer treatments. This transcriptomic analysis should provide insight in the mode of action of the compounds under study and yield generic data relevant to grape defense reaction. The experiment took place in the greenhouse. Six week-herbaceous grape cutting (cv Marselan) were spray-treated with 5 resistance inducers, an adjuvant (used along with some inducers) and water as control. Plants were inoculated by Plasmopara viticola (downy midew) 24h post-treatment. We used 5 plants per treatment. Leaf samples were harvested 24h post-treatment and 48h post-inoculation. Three biological repetitions were done. Each hybridization experiment was performed in comparison with water.
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2024-09-12 | GSE276430 | GEO
Project description:Total RNA was extracted using TRI® Reagent (Sigma). cDNA was synthesized by RevertAid™ First Strand cDNA Synthesis Kit with Oligo dT primers (K1622, Fermentas) following manufacturer’s recommendations. PCR reactions were carried out on a DNAEngine® Thermal Cycler (PTC-0200G, Bio-Rad) in 25 μl reaction volume containing 1 μl cDNA, 200 nM primer pairs and components of TaKaRa Taq™ kit (R001A, Takara). All samples were analyzed in triplicate RT-qPCR.mRNAs were extracted using biotinylated poly(dT) oligo, followed by further removing of contaminated rRNA using RiboMinus Transcriptome Isolation Kit (K1550-02, Invitrogen). Then mRNAs were fragmented into 100-200nt length and subjected to immunoprecipitation with m6A specific antibody.The libraries were sequenced using HiSeq2000 (Illumina) in single-read mode, creating reads with a length of 101 bp. Sequencing chemistry v2 (Illumina) was used and samples were multiplexed in two samples per lane.
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Project description:The transcription factor Max is a basic helix-loop-helix leucine zipper (bHLHLZ) protein that forms homodimers or interacts with other bHLHLZ proteins, including Myc and Mxd proteins. Among this dynamic network of interactions, the Myc/Max heterodimer has crucial roles in regulating normal cellular processes, but its transcriptional activity is deregulated in a majority of human cancers. Despite this significance, the arsenal of high-quality chemical probes to interrogate these proteins remains limited. We utilized small molecule microarrays (SMMs) to identify compounds that bind Max in a mechanistically unbiased manner. We discovered the asymmetric polycyclic lactam, KI-MS2-008, which stabilizes the Max homodimer while reducing Myc protein and Myc-regulated transcript levels. KI-MS2-008 also decreases viable cancer cell growth in a Myc-dependent manner and suppresses tumor growth in vivo. This approach demonstrates the feasibility of modulating Max with small molecules and supports altering Max dimerization as an alternative approach to targeting Myc.
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