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Real-time quantitative PCR analysis of human PBMCS from HBV+ patients and healthy donors

ABSTRACT: Human livers biopsies from HBV+ patients and healthy donors were collected . RNA and Protein were extract and The Human Adipogenesis RT² Profiler™ PCR Array was used to compare the gene expression in the two group. At the same time PBMCs were collected and the same array were used to compare the adipogenesis expression in the two system.

ORGANISM(S): Homo sapiens

PROVIDER: GSE54430 | GEO | 2014/01/28

SECONDARY ACCESSION(S): PRJNA236510

REPOSITORIES: GEO

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Real-time quantitative PCR analysis of human liver biopsies from HBV+ patients and healthy donors

Project description:Human livers biopsies from HBV+ patients and healthy donors were collected . RNA and Protein were extract and The Human Adipogenesis RT² Profiler™ PCR Array was used to compare the gene expression in the two group. At the same time PBMCs were collected and the same array were used to compare the adipogenesis expression in the two system.

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Real-time quantitative PCR analysis of human PBMCS from HBV+ patients and healthy donors

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Real-time quantitative PCR analysis of human PBMCs from HCV+ patients and healthy donors

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Real-time quantitative PCR analysis of human liver biopsies from HBV+ patients and healthy donors

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Project description:Human livers biopsies from HCV+ patients and healthy donors were collected . RNA and Protein were extract and The Human Adipogenesis RTM-BM-2 ProfilerM-bM-^DM-" PCR ArrayM-BM- was used to compare the gene expression in the two group. At the same time PBMCs were collected and the same array were used to compare the adipogenesis expression in the two system. qPCR gene expression profiling. Fresh Livers from 40 HBV+ and 20 HD patients were used . Equal amount total RNA from each samples were used.

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Project description:huh7.5 hepatic cell lines were infected with HCV. RNA and Protein were extract after 72 hours and The Human Adipogenesis RT² Profiler™ PCR Array was used to compare the gene expression in the two group.

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