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Downregulation of micro RNAs 145-3p and 145-5p is a Long-Term Predictor of Postmenopausal Breast Cancer Risk: the ORDET prospective study

ABSTRACT: microRNA expression profile of buffy coat derived from healty woman before the insorgence of breast cancer

ORGANISM(S): Homo sapiens

PROVIDER: GSE54470 | GEO | 2014/09/10

SECONDARY ACCESSION(S): PRJNA236592

REPOSITORIES: GEO

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Downregulation of micro RNAs 145-3p and 145-5p is a Long-Term Predictor of Postmenopausal Breast Cancer Risk: the ORDET prospective study

Project description:microRNA expression profile of buffy coat derived from healty woman before the insorgence of breast cancer microRNA expression was evaluated from total RNA extracted from buffy coat.

2014-09-10 | E-GEOD-54470 | biostudies-arrayexpress

Downregulation of micro RNAs 145-3p and 145-5p is a Long-Term Predictor of Postmenopausal Breast Cancer Risk: the ORDET prospective study

| S-ECPF-GEOD-54470 | biostudies-other

Cord blood buffy coat DNA methylation is comparable to whole cord blood methylation

Project description:Cord blood DNA methylation is associated with numerous health outcomes and environmental exposures. Whole cord blood DNA reflects all nucleated blood cell types, while centrifuging whole blood separates red blood cells by generating a white blood cell buffy coat. Both sample types are used in DNA methylation studies. Cell types have unique methylation patterns and processing can impact cell distributions, which may influence comparability. To evaluate differences in cell composition and DNA methylation between buffy coat and whole cord blood samples, cord blood DNA methylation was measured with the Infinium EPIC BeadChip (Illumina) in 8 individuals, each contributing buffy coat and whole blood samples.

2018-01-01 | GSE103189 | GEO

Smaller stature in childhood following assisted reproductive technologies (ART) is not explained by parental or gestational factors, early childhood environment, or fetal DNA methylation [GUSTO_buffy_coat]

Project description:As part of an investigation on contributors to offspring size following assisted reproduction, we investigated maternal mid-/late-pregnancy peripheral blood buffy coat DNA methylation at 240 CpGs previously associated with maternal pregnancy morbidity or offspring growth across various tissues. Illumina Infinium MethylationEPIC BeadChip was used to obtain DNA methylation profiles in previously frozen buffy coat samples from 70 women who conceived via assisted reproduction technologies and gave live births and 845 women who spotaneously conceived and gave live births.

2020-09-16 | GSE158063 | GEO

Human buffy coat gene expression, custom 250-plex Nanostring panel

Project description:Buffy coat PBMC RNA expression was tested using a custom Nanostring probe panel, to identify general immune and glucocorticoid receptor-associated immune genes. ClinicalTrials.gov Identifier: NCT00824941

2019-11-01 | GSE124549 | GEO

Single cell RNAseq reveal covid19 patient immune response

Project description:Eighteen patients admitted to CSMC and diagnosed with COVID19 by RT-PCR were stratified into COVID19 mild/moderate, severe, and recovery groups (n=5-6/group). Venous blood was collected into EDTA coated tubes and centrifuged to separate plasma and buffy coat. Plasma was collected and frozen at -80C, and the buffy coat was collected into cryo-preservation media and frozen at -80C. Recovered cells are sorted for live-dead staining, then fixed with methanol. Fixed single cells were further captured using 10x chromium Next GEM 3 prime v3.1 kit. Two patients samples from the same group were mixed, captured and sequenced together.

2020-09-24 | GSE154567 | GEO

A comprehensive gene profile investigation of the patients with high risk etiology of colorectal cancer

Project description:Interventions: We perform fenetic analysis of tumor, non-tumor, plasma, buffy coat of patients of colorectal cancer. Primary outcome(s): Somatic and germline mutation of cororeactal cancer. Study Design: Single arm Non-randomized

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Global Gene Expression Profiling of Bovine Lymphocyte Populations

Project description:Gene expression profiling of pooled lymphocyte populations from clinically normal cattle. The T lymphocyte populations were purified from buffy coat and the immature B cells were prepared from isolated Peyer's patch follicles. Keywords: other

2005-03-31 | GSE2244 | GEO

DNA methylation profiles of human buffy coat samples from Huntington's disease

Project description:Human DNA methylation Beadchip v1.2 was used to profile buffy coat samples. The main goal of the study was to relate DNA methylation data to Huntington disease status (control, pre-manifest disease, manifest motor disease).

2020-07-02 | GSE146917 | GEO

Burn/Trauma Study

Project description:#G017 Burn/Trauma Study. This is a Glue Grant Study comparison between gene expression analyses performed in eight trauma/burn subjects using either a buffy coat isolation of whole blood or using the PAXgene system Keywords: other

2004-04-24 | GSE1343 | GEO

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