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Diurnal variation for rice leaves in a growth chamber

ABSTRACT: Time-course transcriptional profiling to compare progression of subjective time with that in the field. The subjective time was inferred from expressions of multiple circadian clock-related genes. 24-h time series with 2-h interval were sampled for 2 developmental stage with no replicate. Two more varieties were sampled only at 3 time points for plants of 28 days past sown (DPS).

ORGANISM(S): Oryza sativa

PROVIDER: GSE54525 | GEO | 2015/03/10

SECONDARY ACCESSION(S): PRJNA236773

REPOSITORIES: GEO

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Diurnal variation for rice leaves in a growth chamber

Project description:Time-course transcriptional profiling to compare progression of subjective time with that in the field. The subjective time was inferred from expressions of multiple circadian clock-related genes. 24-h time series with 2-h interval were sampled for 2 developmental stage with no replicate. Two more varieties were sampled only at 3 time points for plants of 28 days past sown (DPS). Jun, Matsuzaki

2015-03-10 | E-GEOD-54525 | biostudies-arrayexpress

Time-course in rice leaves in the field: WT (cv.Norin8) vs. osgi mutant

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The basal cm of leaf 4 of GA2OX::PLA1 (193-01) versus wild type in the growth chamber and the field (May-June 2015)

Project description:GA2OX::PLA1 transgenic plants and their none-transgenic siblings were grown in the field in Wetteren and in the growth chamber. In the field, we sampled three plants per plot (three plots were sown per genotype), resulting in three pools each consisting of three plants. Every pool represented a different subplot. In the growth chamber, the plants were sampled randomly across the population, with three pools, each consisting of three plants. For each plant, the day of leaf 4 appearance was marked and the samples were taken two days after leaf 4 appeared (DALA). The seedlings were cut just above soil level and the growing fourth leaf was dissected out and the younger leaves inside leaf 4 were removed. Using a ruler we sampled the basal cm of leaf 4 (division zone) for RNA preparation. The samples were snap frozen and transported to the lab for storage at -80 degrees and further processing. One time point was sampled, two days after leaf 4 appearance.

2019-10-16 | E-MTAB-8094 | biostudies-arrayexpress

The basal cm of leaf 4 of GA2OX::PLA1 (193-01) versus wild type in the growth chamber and the field (May-June 2016)

Project description:GA2OX::PLA1 transgenic plants and their none-transgenic siblings were grown in the field in Wetteren and in the growth chamber. In the field, we sampled three plants per plot (three plots were sown per genotype), resulting in three pools each consisting of three plants. Every pool represented a different subplot. In the growth chamber, the plants were sampled randomly across the population, with three pools, each consisting of three plants. For each plant, the day of leaf 4 appearance was marked and the samples were taken two days after leaf 4 appeared. The seedlings were cut just above soil level and the growing fourth leaf was dissected out and the younger leaves inside leaf 4 were removed. Using a ruler we sampled the basal cm of leaf 4 (division zone) for RNA preparation. The samples were snap frozen and transported to the lab for storage at -80 degrees and further processing. Two time points were sampled, two days after leaf 4 appearance as well as six days after leaf appearance.

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Circadian and diurnal expression data of Synechococcus

Project description:In the unicellular cyanobacterium, Synechococcus elongatus PCC 7942, essentially all promoter activities are under the control of the circadian clock in continuous light (LL) conditions. Here, we employed high-density oligonucleotide arrays to explore comprehensive profiles of genome-wide Synechococcus gene expression in wild type, kaiABC-null and kaiC-overexpressor strains under LL and continuous dark (DD) conditions. In the wild type strain more than 30% of transcripts significantly oscillated in a circadian fashion, peaking at subjective dawn and dusk. Such circadian control was nullified in kaiABC-null strains. Although KaiC has been proposed to globally repress gene expression, our analysis revealed that dawn expressing genes were upregulated by kaiC-overexpression, such that the clock was arrested at subjective dawn. Transfer of cells to continuous dark (DD) conditions from LL immediately suppressed expression of most of genes, while the clock keeps time even in the absence of transcriptional feedback. Thus, the Synechococcus genome seems primarily regulated by the light/dark cycles and dramatically modified by the protein-based circadian oscillator. Keywords: timecourse data (~48 hours under continuous light or darkness) from Synechococcus elongatus PCC 7942 (wild type, kaiABC-null, and inducible kaiC-overexpressor) strains

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Diurnal Regulation of Gene Expression in Leaves Over a 72-Hour Period

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2010-09-02 | E-GEOD-23917 | biostudies-arrayexpress

Analysis of gene expression in grape berries due to diurnal effects (DIU_05).

Project description:This is a 24 hour time course experiment to investigate changes in gene expression in deseeded berries sampled every three hours within a 24 hour period at 2 time points during development i.e. T5 (preveraison) & T17 (postveraison). Changes in gene expression within the 24 hour period were analyzed to identify expression profiles that coincided with diurnal changes. The gene expression profiles were then to be compared between the two developmental time points T5 v T17). Keywords: Diurnal time course

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Analysis of gene expression in grape berries due to diurnal effects (DIU_06).

Project description:This is a 24 hour time course experiment to investigate changes in gene expression in deseeded berries sampled every three hours within a 24 hour period at 2 time points during development i.e. T4 (preveraison) & T15 (postveraison). Changes in gene expression within the 24 hour period were analyzed to identify expression profiles that coincided with diurnal changes. The gene expression profiles were then to be compared between the two developmental time points T4 v T15). Keywords: Diurnal time course

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2015-03-31 | GSE67434 | GEO

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