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ChIP-seq mapping of histone modifications in CD14++ CD16- monocytes from human blood samples

ABSTRACT: Chromatin immunoprecipitation in combination with a genome-wide analysis via high-throughput sequencing is the state of the art method to gain genome-wide representation of histone modification or transcription factor binding profiles. However, chromatin immunoprecipitation analysis in the context of human experimental samples is limited, especially in the case of blood cells. The typically extremely low yields of precipitated DNA are usually not compatible with library amplification for next generation sequencing. We developed a highly reproducible protocol to present a guideline from the first step of isolating monocytes from a blood sample to analyse the distribution of histone modifications in a genome-wide manner.

ORGANISM(S): Homo sapiens

PROVIDER: GSE54634 | GEO | 2014/09/01

SECONDARY ACCESSION(S): PRJNA237233

REPOSITORIES: GEO

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ChIP-seq mapping of histone modifications in CD14++ CD16- monocytes from human blood samples

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