Transcriptomics

Dataset Information

0

Glucocorticoid induced gene-regulation in murine bone marrow derived monocytes.


ABSTRACT: Glucocorticoids (GC) are used as first line therapies for generalized suppression of inflammation (e.g. allergies or autoimmune diseases), but their long-term use is limited by severe side effects. Our previous work has revealed that GC induced a stable anti-inflammatory phenotype in monocytes, the glucocorticoid-stimulated monocytes (GCsM) that we now exploited for targeted GC-mediated therapeutic effects. We demonstrate that GCsM interact with T-cells in suppressing proliferation as well as cytokine release of CD8+ and especially CD4+ T-cells in vitro, and that they support generation of Foxp3+ cells. We therefore tested their immunosuppressive potential in CD4+ T-cell-induced colitis in vivo. We found that injection of GCsM into mice with already established severe colitis abolishes the inflammation and results in significant clinical improvement within a few days. T-cells recovered from GCsM-treated mice reveal reduced secretion of pro-inflammatory cytokines IFN- or IL-17. Furthermore, accumulation of clusters of Foxp3+ CD4+ T-cells were detectable at local sites of inflammation in their colon. Thus, GCsM are able to modify T-cell responses in vitro and in vivo and are able to down-regulate and clinically cure an established severe T-cell mediated colitis.

ORGANISM(S): Mus musculus

PROVIDER: GSE54778 | GEO | 2014/09/02

SECONDARY ACCESSION(S): PRJNA237608

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2014-09-02 | E-GEOD-54778 | biostudies-arrayexpress
2017-11-03 | PXD007725 | Pride
2022-05-03 | GSE169006 | GEO
2010-07-08 | E-GEOD-20523 | biostudies-arrayexpress
2024-08-16 | PXD054940 |
2021-07-28 | GSE168218 | GEO
2021-07-28 | GSE168217 | GEO
2021-07-28 | GSE168216 | GEO
2008-08-21 | GSE12506 | GEO
2008-10-25 | E-GEOD-12506 | biostudies-arrayexpress