Transcriptomics

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RNAseq of memory cocktail versus IL-2 grown OT-I CD8 T cells


ABSTRACT: Cytotoxic T cells are typically expanded ex vivo for adoptive immunotherapy by culture with IL-2. This culture period leads to a differentiated phenotype and acquisition of effector function, as well as a loss of in vivo proliferative capability and anti-tumor efficacy. Here, we report antigen-specific and polyclonal expansion of cytotoxic T cells in a cocktail of cytokines and small molecules that leads to a memory-like phenotype in mouse and human cells even during extended culture, leading to enhanced in vivo expansion and tumor control.

ORGANISM(S): Mus musculus

PROVIDER: GSE54877 | GEO | 2014/08/12

SECONDARY ACCESSION(S): PRJNA237954

REPOSITORIES: GEO

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