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BTG1 and BTG2 Regulate B Linege Commitment by Modulation of EBF1 Activity

ABSTRACT: The human BTG1 and BTG2 genes are targeted by genetic alterations in hematological malignancies. In this study we examined the unique and overlapping functions of Btg1 and Btg2 in mouse B cell development. Mice deficient for Btg1 and Btg2 displayed the strongest phenotype with a decrease in B cell numbers in bone marrow due to impaired B cell commitment and immature B cell differentiation. To determine the underlying mechanism, microarray expression analyis was performed on early (B220+ CD43+) and late (B220+ CD43-) B cells of WT and Btg1;Btg2 dKO mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE55997 | GEO | 2014/03/19

SECONDARY ACCESSION(S): PRJNA242137

REPOSITORIES: GEO

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BTG1 and BTG2 Regulate B Linege Commitment by Modulation of EBF1 Activity

Project description:The human BTG1 and BTG2 genes are targeted by genetic alterations in hematological malignancies. In this study we examined the unique and overlapping functions of Btg1 and Btg2 in mouse B cell development. Mice deficient for Btg1 and Btg2 displayed the strongest phenotype with a decrease in B cell numbers in bone marrow due to impaired B cell commitment and immature B cell differentiation. To determine the underlying mechanism, microarray expression analyis was performed on early (B220+ CD43+) and late (B220+ CD43-) B cells of WT and Btg1;Btg2 dKO mice. Total RNA obtained from sorted B220+ CD43+ ( Hardy fractions ABC) and B220+ CD43- (Hardy fractions DEF) BM cells of WT and Btg1-/-;Btg2-/- mice. In total 4 samples were analyzed and no replicates were included.

2014-03-19 | E-GEOD-55997 | biostudies-arrayexpress

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mRNA destabilization by BTG1 and BTG2 maintains T cell quiescence [RNA-seq]

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BTG1 regulates glucocorticoid receptor autoinduction in acute lymphoblastic leukemia

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BTG1 mutation yields super-competitive B cells primed for malignant transformation

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