Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

NRG-1 induces mature ventricular cardiac differentiation from iPS cells contributing to cardiac tissue repair

ABSTRACT: Combined treatment with NRG-1 and DMSO led to efficient differentiation of iPS into mature ventricular-like cardiac cells, which were capable of preserving cardiac function and tissue viability when transplanted into a mouse model of myocardial infarction.

ORGANISM(S): Mus musculus

PROVIDER: GSE56511 | GEO | 2017/03/24

SECONDARY ACCESSION(S): PRJNA243570

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Characterization of the effect of overexpressing the Nrg-1 (neuregulin-1) gene, using the cardiac driver line Nkx2.5-Cre, in E (embryonic day) 15.5 heart venticles

Project description:To investigate the role of Nrg-1 in heart ventricular chamber development, we overexpressed Nrg-1 in cardiac lineages, harvested ventricles at E15.5, and determined gene expression profiles, using RNA-seq.

2023-12-01 | GSE216467 | GEO

Role of Nrg-1 in the development of heart ventricular chamber in mice.

Project description:To investigate the role of Nrg-1 in heart ventricular chamber development, we have used RNA-Seq to characterize the effect of Nrg-1 inactivation or over-expression in mouse embryos at E9.5 and E15.5. This SuperSeries is composed of the SubSeries listed below.

2023-12-01 | GSE216471 | GEO

Infarcted rats teated with rhNRG-1(recombinant human NRG-1)

2007-10-18 | GSE9263 | GEO

Direct in vivo cardiac reprogramming improves cardiac function and ventricular remodeling in chronic MI.

Project description:Heart failure (HF) is a leading cause of morbidity and mortality. As adult cardiomyocytes (CMs) have little regenerative capacity, after myocardial infarction (MI), resident cardiac fibroblasts (CFs) synthesize extracellular matrix to form scar tissues, resulting in myocardial remodeling and HF. Thus, both cardiac regeneration and fibrosis are therapeutic targets for chronic MI. We previously reported that fibroblasts were directly reprogrammed into induced CMs (iCMs) by overexpression of cardiogenic transcription factors in acute and chronic MI. Here we show that in vivo cardiac reprogramming improved cardiac function, and reversed cardiac remodeling in chronic MI using a novel transgenic mouse system. Transcriptome analysis revealed that in vivo cardiac reprogramming suppressed signs of fibrosis and inflammation. Thus, in vivo cardiac reprogramming may be a promising approach for chronic HF.

2024-07-01 | GSE240310 | GEO

Neuregulin-1? induces mature ventricular cardiac differentiation from induced pluripotent stem cells contributing to cardiac tissue repair.

Project description:Stem cell-derived cardiomyocytes (CMs) are often electrophysiologically immature and heterogeneous, which represents a major barrier to their in vitro and in vivo application. Therefore, the purpose of this study was to examine whether Neuregulin-1? (NRG-1?) treatment could enhance in vitro generation of mature "working-type" CMs from induced pluripotent stem (iPS) cells and assess the regenerative effects of these CMs on cardiac tissue after acute myocardial infarction (AMI). With that purpose, adult mouse fibroblast-derived iPS from ?-MHC-GFP mice were derived and differentiated into CMs through NRG-1? and/or dimethyl sulfoxide (DMSO) treatment. Cardiac specification and maturation of the iPS was analyzed by gene expression array, quantitative real-time polymerase chain reaction, immunofluorescence, electron microscopy, and patch-clamp techniques. In vivo, the iPS-derived CMs or culture medium control were injected into the peri-infarct region of hearts after coronary artery ligation, and functional and histology changes were assessed from 1 to 8 weeks post-transplantation. On differentiation, the iPS displayed early and robust in vitro cardiogenesis, expressing cardiac-specific genes and proteins. More importantly, electrophysiological studies demonstrated that a more mature ventricular-like cardiac phenotype was achieved when cells were treated with NRG-1? and DMSO compared with DMSO alone. Furthermore, in vivo studies demonstrated that iPS-derived CMs were able to engraft and electromechanically couple to heart tissue, ultimately preserving cardiac function and inducing adequate heart tissue remodeling. In conclusion, we have demonstrated that combined treatment with NRG-1? and DMSO leads to efficient differentiation of iPS into ventricular-like cardiac cells with a higher degree of maturation, which are capable of preserving cardiac function and tissue viability when transplanted into a mouse model of AMI.

| S-EPMC4313422 | biostudies-literature

Expression data from MCF7 cells treated with Neuregulin (NRG) at different times

Project description:To gain insights into the mechanism responsible for the protumorigenic actions of NRG we performed gene expression analyses of MCF7 cells treated with soluble NRG for 3, 6, 12 and 24 hours. MCF7 cells were treated with NRG for 0, 3, 6,12, 24 hours, 3 for each condition, prepared from three different experiments

2015-06-04 | E-GEOD-69540 | biostudies-arrayexpress

Expression data from MCF7 cells treated with Neuregulin (NRG) at different times

2015-06-04 | GSE69540 | GEO

Mouse Cardiac Fibroblasts post MI

Project description:Analysis of one-day cultured cardiac fibroblasts after acute myocardial infarction

2020-05-20 | MSV000085454 | MassIVE

Transcription profiling of infarcted rats teated with rhNRG-1(recombinant human NRG-1)

Project description:The ErbB famliy of erceptor tyrosine kinases and their ligands, neuregulins(NRGs), are crtical involved not only in cardiac development but also in the maintenace of structural and functional integrity of the adult heart. rhNRG-1(recombinant human NRG-1) Improves cardiac function and sruvival in models of ischemic, dilated, and viral cardiomyopathy with the ErbB-activation. But the mechanism is still unknown Experiment Overall Design: Three individual samples in each group (vehicle, rhNRG-1 treated or sham operated rats) were hybridized with 9 GeneChipÂ® Rat Genome 230 2.0 Arrays (Affymetrix) separately.A total of 1950 genes (at least a 1.5-fold change in expression level compared with any another groups, all p <0.05),were selected and used as input for hierarchical clustering, a computational method that groups experimental samples according to similarity in patterns of gene expression across a large number of genes.

2008-06-16 | E-GEOD-9263 | biostudies-arrayexpress

Expression data from GGF2-treated post-MI swine

Project description:Neuregulin-1 (NRG-1) is a paracrine factor critical for cardiac development. We have been examining whether the recombinant NRG-1β isoform known as glial growth factor 2 (GGF2) has therapeutic potential for heart failure. In both small and large animals after experimental myocardial infarction (MI) we have found that GGF2 treatment improves myocardial function and limits progressive myocardial remodeling. To understand potential mechanisms for this effect, we compared gene expression in swine by microarray analysis. We used microarrays to compared th the global gene expression underlying the efficacy of GGF2 treatment for heart injury.

2014-06-21 | GSE48255 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data