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Genome-wide expression profiling in bone marrow-derived macrophage (BMM) cells upon RANKL treatment

ABSTRACT: To screen for altered gene expression during osteoclastogenesis, RANKL-treated BMM cells were subjected to gene expression profiling.

ORGANISM(S): Mus musculus

PROVIDER: GSE57468 | GEO | 2014/07/30

SECONDARY ACCESSION(S): PRJNA246517

REPOSITORIES: GEO

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Genome-wide expression profiling in bone marrow-derived macrophage (BMM) cells upon RANKL treatment

Project description:To screen for altered gene expression during osteoclastogenesis, RANKL-treated BMM cells were subjected to gene expression profiling. BMM cells were treated with M-CSF and RANKL for 0, 1, 2 and 3 days. Total RNA was isolated and analyzed by gene expression microarray using the MouseRef-8 v2.0 Expression BeadChip.

2014-07-30 | E-GEOD-57468 | biostudies-arrayexpress

Abeliophyllum distichum Nakai Extract exerts anti-osteoporotic effects by suppressing RANKL-induced osteoclastogenesis

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Project description:To screen for altered gene expression during osteoclastogenesis, BMM cells depleted of TMBIM6 with or without RANKL were subjected to gene expression profiling.

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Murine Bone Marrow Macrophages: Control vs. RANKL Stimulated

Project description:To identify the microRNAs that are involved in osteoclastogenesis, microRNA expression profiles in mouse bone marrow macrophages (BMMs) stimulated with RANKL (BMOc) were compared with that of control untreated BMMs. These results provide insights into the mechanisms to regulate osteoclastogenesis and bone resorption activities in osteoclasts by microRNA. BMMs were cultured with 20 ng/ml M-CSF in the presence or absence of 50 ng/ml RANKL for 24 hours. Cells were collected for total RNA isolation, and were subjected to microRNA array analysis.

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RankL induced mice BMM treated with or without dimethyl itaconate for 24h

Project description:RankL induced mice BMM following dimethyl itaconate treatment for 24h

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Ethyl acetate fraction of Lentinula edodes (LEA) inhibits osteoclastogenesis by suppressing NFATc1 expression

Project description:To screen for altered gene expression during osteoclastogenesis, BMM cells treated with RANKL or RANKL+LEA were subjected to gene expression profiling.

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Murine Bone Marrow Macrophages: Control vs. RANKL Stimulated

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Transcription profiling of bone marrow macrophages (BMM), BMM co-cultured with breast cancer cell line 4T1.2, and RAW macrophage cell line

Project description:Exon ST arrays were used to compare gene expression in bone marrow macrophages (BMM), BMM co-cultured with breast cancer cell line 4T1.2, RAW macrophage cell lines, and tumour supernatant.

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RankL induced mice BMM treated with or without dimethyl itaconate for 24h

Project description:RankL induced mice BMM treated with or without dimethyl itaconate for 24h

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Profiling of DNA Methylation during RANKL Induced Osteoclastogenesis using Whole-Genome Bisulfite Sequencing

Project description:Purpose: The goals of this study are to describe the DNA methylation profile during RANKL induced osteoclastogenesis. Methods: Primary bone marrow cells were isolated and treated with M-CSF (30ng/mL) to generate bone marrow macrophages (BMMs). BMMs were then used to generate preosteoclast (pOC) stimulated by M-CSF (30ng/mL) together with RANKL (100ng/mL) for 24 h and mature osteoclast (mOC) stimulated by M-CSF (30ng/mL) together with RANKL (100ng/mL) for 72 h or more. DNA methylation profiles of BMM, pOC and mOC were generated by deep sequencing, using Illumina NovaSeq 6000. Results: After acquiring of clean reads, we used Hisat2 for spliced mapping. We mapped about 40 million sequence reads per sample of the WGBS results. Saturation rate analysis was then performed evaluating the gene coverage rate along with read depth increase. The transcripts mapping to difference functional region of the genome was then analyzed. Circos software was used to map the consistency of samples with mouse genome on a chromosomal level. The quantification of transcript intensity was analyzed using RPKM (Reads Per Kb per Million reads). Conclusions: Our study represents the first high-throughput WGBS sequencing data of DNA methylation during osteoclastogenesis.

2020-06-04 | GSE133515 | GEO

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