Transcriptomics

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Impacts of high ATP supply from chloroplasts and mitochondria on the leaf metabolism of Arabidopsis thaliana


ABSTRACT: Arabidopsis thaliana ecotypes Columbia (overexpression line) was used in this study. After sterilization, the seeds were placed on Murashige and Skoog medium supplemented with 2% (w/v) sucrose for 10 days and then the seedling were transferred to soil under 16 hours light (22°C) / 8 hours dark (18°C) period in growth chamber at a light intensity of 120?150 µmol m-2 s-1. 20-day-old Arabidopsis leaves without bolting were immediately frozen in liquid nitrogen for RNA and protein and metabolites extraction. Leaves were harvested at three different time points: t = 0 hr (end of night), t = 1 hr (one hour after light turn on) and t = 8 hr (eight hours after light turn on), respectively.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE57790 | GEO | 2016/01/21

SECONDARY ACCESSION(S): PRJNA248110

REPOSITORIES: GEO

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