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Zebrafish immune response to bacterial and viral infection

ABSTRACT: In order to explore the relative importance of adult zebrafish innate and adaptive immune responses to different kinds of infections, we compared the gene expression profile of VHSV-challenge-survivors, bacterial infection survivors and control non infected zebrafish.

ORGANISM(S): Danio rerio

PROVIDER: GSE57952 | GEO | 2015/05/12

SECONDARY ACCESSION(S): PRJNA248479

REPOSITORIES: GEO

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Zebrafish immune response to bacterial and viral infection

Project description:In order to explore the relative importance of adult zebrafish innate and adaptive immune responses to different kinds of infections, we compared the gene expression profile of VHSV-challenge-survivors, bacterial infection survivors and control non infected zebrafish. VHSV -infection-survivors zebrafish were divided in two groups of 12 individuals, first group was infected with VHSV (samples named A) and second group was mock-infected (samples named B). Two days after challenge, zebrafish of each group were divided in four subgroups (3 fishes per group) and head kidney and spleen of each individual were sampled. For each group, internal organs of three fishes were pooled, so four biological replicates were generated . Zebrafish surviving a natural infection caused by Aeromonas hydrophila and Vibrio fluvialis (samples named C) were divided in four groups (3 fishes per group) and head kidney and spleen were extracted. Finally, 12 healthy zebrafish were used as control group (samples named D) and processed in the same way as group C.

2015-05-12 | E-GEOD-57952 | biostudies-arrayexpress

Zebrafish early transcriptional response to viral haemorrhagic septicemia rhabdovirus (VHSV) challenge vs2

Project description:We describe here the transcripts induced after infection of zebrafish with viral haemorrhagic septicemia rhabdovirus (VHSV). Two days after infection, differentially expressed transcript levels from selected immune-related zebrafish genes were studied in both fins and internal organs (pooled spleen, head kidney and liver). VHSV-induced gene expression in zebrafish fins and organs was measured at 2 days after infection. Four independent experiments were performed for each treatment and their corresponding controls

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Gene expression profiles after infection of zebrafish with viral haemorrhagic septicemia rhabdovirus (VHSV)

Project description:We describe for the first time transcripts induced after infection of zebrafish with viral haemorrhagic septicemia rhabdovirus (VHSV). Two days after infection, differentially expressed transcript levels from selected immune-related zebrafish genes were studied in both fins and internal organs (pooled spleen, head kidney and liver).

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Study of the differentially-expressed genes induced by the expession of two viral glycoproteins in muscle cells of adult zebrafish

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Gene expression profiles after injection of zebrafish with NV (non virion) recombinant protein from Viral Hemorrhagic Septicemia Virus (VHSV)

Project description:NV is a viral protein only present in the genus Novirhabdovirus (fam Rhabdoviridae), its function is not well characterized yet. In this study we try to shed some light in the biological function of this viral protein in the zebrafish. We describe here transcripts induced after injection of adult zebrafish with NV recombinant protein of VHSV. Two days after infection, differentially expressed transcript levels from selected immune-related zebrafish genes were studied in zebrafish internal organs (pooled spleen and head kidney).

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Gene expression profile after Spring Viraemia Carpae Virus (SVCV) infection in zebrafish mutants rag1 -/- versus wt

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Specialized reaction to bacterial infection precedes antiviral innate immunity during development of teleost fish

Project description:During early stages of development, juvenile fish must rely on their innate immune system to defend against pathogens. At these early stages, the immune system is immature and is unlikely to express the full repertoire of genes that control defences. Although vulnerable, these larval fish can still fight of infections, indicating there are active defence mechanisms. Using rainbow trout as a model, we have taken a transcriptomics approach to determine the antibacterial (Aeromonas salmonicida) and antiviral (VHSV) responses to infection at four early life history stages, eyed egg, post-hatch, first feeding and three weeks post-first feeding. We performed microarray analysis using an Agilent 4x44K custom designed array, using a common RNA reference hybridization design. We show that all stages of the developing fish respond to the disease challenge at 3 days post-challenge, but the number and complexity of the response increases with developmental stage. Specifically, the response to virus at eyed egg and hatch stages does not show the full interferon response as is found at first feeding and 3 weeks post-first feeding. The experiments were carried out with a custom-designed Agilent (Agilent design ID: 028918) oligonucleotide microarray (A-MEXP-2315) named Trout_imm_v1. In total, 46 hybridisations were performed on individual animals, with 4 biological replicates per sampling point (apart from Bacteria infected eyed eggs for which two hybridizations were not used). This work was funded by the European Community’s seventh framework programme (FP7/2007-2013), under grant agreement no. 222719 (LIFECYCLE).

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