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CGH: FN1.4_v_M92-220

ABSTRACT: Soybean fast neutron (FN) mutant lines were compared by CGH to catalog structurally variant (e.g. deletion and duplication) regions compared to the parent line (M92-220). The CGH comparisons reveal reveal putative deletions and duplications among the mutant lines.

ORGANISM(S): Glycine max

PROVIDER: GSE58172 | GEO | 2014/06/04

SECONDARY ACCESSION(S): PRJNA251560

REPOSITORIES: GEO

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CGH: FN1.4_v_M92-220

Project description:Soybean fast neutron (FN) mutant lines were compared by CGH to catalog structurally variant (e.g. deletion and duplication) regions compared to the parent line (M92-220). The CGH comparisons reveal reveal putative deletions and duplications among the mutant lines. FN lines were compared through a common reference, M92-220 (this is a sub-line individual isolated from the M92-220 seed stock). Each FN line was represented by a single individual plant. The reported values show the log2 ratio of the normalized FN line hybridization signals (Cy3) over the normalized M92-220 hybridization signal (Cy5).

2014-06-04 | E-GEOD-58172 | biostudies-arrayexpress

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Soybean fast neutron genotyping: 700k Forward genetics

Project description:CGH was used to compare induced structural variation among soybean fast neutron lines, developed in background M92-220. 114 different soybean fast neutron lines were hybridized against the parental reference genotype M92-220. The soybean tiling array consists of 700k probes, spaced at approximately 1.1 kb intervals.

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2011-08-02 | E-GEOD-31111 | biostudies-arrayexpress

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Project description:CGH was used to compare induced structural variation among soybean fast neutron lines, developed in background M92-220.

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Seed composition traits: comparative genomic hybridization analysis in glycine max fast neutron mutants

Project description:Ten mutants with altered seed composition traits were identified in a soybean fast neutron population (Bolon et al. 2014). These mutant lines were maintained to an advanced generation (ranging between M5 and M9) and compared to their wild-type parent (M92-220-Long) using CGH to identify the causative region/gene associated with the seed composition changes.

2018-08-16 | GSE118594 | GEO

High-sucrose/low-oil comparative genomic hybridization analysis in glycine max fast neutron mutants

Project description:Two high-sucrose/low-oil mutants (FN0176450/2012CM7F040P06/SRX826343 and FN0176450/2012CM7F040P05/SRX826351) were identified in a soybean fast neutron population (Bolon et al. 2014). These mutant lines were then advanced to the M3:7 generation and compared to thier wild-type parent (M92-220-Long) using CGH to identify the causative region/gene associated with high-sucrose/low-oil.

2017-01-14 | GSE93411 | GEO

Short trichome comparative genomic hybridization analysis in glycine max fast neutron mutants

Project description:One short trichome mutant (family known by multiple names, including R59C46, FN0175946, and FNMN0409) was identified in a soybean fast neutron population (known as VP02 in Bolon et al. 2011). An advanced line of this mutant was compared to its wild-type parent (M92-220-Long) using CGH to identify the causative region/gene associated with the short trichome phenotype.

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CGH: Transgenic soybeans and controls

Project description:Transgenic soybean lines were compared to their parental lines by CGH to catalog structurally variant (e.g. deletion and duplication) regions among these genotypes. The CGH comparisons reveal reveal putative deletions and duplications among the 5 lines.

2015-09-30 | GSE73596 | GEO

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