PROTEOFORMER: deep proteome coverage through ribosome profiling and MS integration
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ABSTRACT: An increasing amount of studies integrate mRNA sequencing data into MS-based proteomics to complement the translation product search space. We present the generation of a protein synthesis-based database from deep sequencing of ribosome-protected mRNA fragments. This approach increases the overall protein identification rates with 3% and 11% (improved and new identifications) for human and mouse respectively and enables proteome-wide detection of 5’-extended proteoforms, uORF translation and near-cognate translation start sites.
ORGANISM(S): Homo sapiens
PROVIDER: GSE58207 | GEO | 2014/12/01
SECONDARY ACCESSION(S): PRJNA251587
REPOSITORIES: GEO
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