Genomics

Dataset Information

0

Activation of a SOX2-dependent transcriptional regulatory circuit drives glioblastoma.


ABSTRACT: Overexpression of the transcription factor SRY-related box 2 (SOX2) is characteristic of Glioblastoma (GBM), yet its regulatory network in vivo is poorly understood. Using a human orthotopic tumor model of GBM for ChIP-Seq analysis, we mapped the SOX2 cistrome. Integrative analysis of SOX2 cistrome and GBM transcriptome data identified two transcription factors, oligodendrocyte lineage transcription factor 2 (OLIG2) and zinc finger E-box binding homeobox 1 (ZEB1) as critical SOX2 targets. Their expression is strongly correlated with SOX2 expression in clinical GBM specimens, and all three proteins are frequently co-expressed in primary Glioblastoma cells, even in the setting of EGFR intratumoral heterogeneity. Sox2, Olig2, and Zeb1 expression is activated by the oncogene EGFRvIII in a murine glioma model, and we demonstrate that co-expression of these transcription factors transforms tumor suppressor deficient astrocytes in the absence of an upstream oncogene and that the resulting tumors exhibit the major histopathological features of GBM.

ORGANISM(S): Homo sapiens

PROVIDER: GSE58345 | GEO | 2017/01/24

SECONDARY ACCESSION(S): PRJNA252047

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2014-04-10 | E-GEOD-54047 | biostudies-arrayexpress
2018-07-30 | PXD008311 | Pride
2013-01-01 | E-GEOD-36900 | biostudies-arrayexpress
2015-09-14 | E-GEOD-72468 | biostudies-arrayexpress
2015-09-08 | E-GEOD-51062 | biostudies-arrayexpress
2014-04-14 | E-GEOD-54791 | biostudies-arrayexpress
2013-01-01 | E-GEOD-36901 | biostudies-arrayexpress
2024-04-09 | PXD050279 | Pride
2014-06-04 | E-GEOD-44561 | biostudies-arrayexpress
2013-01-01 | GSE36901 | GEO