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Transcriptomic analysis of two 14-3-3 proteins Bmh1 and Bmh2 under osmotic and oxidative stresses in the entomopathogen fungus Beauveria bassiana by using RNA sequencing

ABSTRACT: Bmh1 and Bmh2 contribute equally to mediating fungal stress tolerance of Beauveria bassiana , a determinant to the biocontrol potential of fungal entomopathogens. We characterize their functionsfor the first time and interaction by multi-phenotypic analyses of single-gene deletion mutants and double-gene mutants by RNAi, and to probe possible mechanisms involved in multi-phenotypic changes by analysis of Bmh1- and Bmh2-specific transcriptomes constructed under osmotic and oxidative stresses versus wild-type (BbWT) through using high throughput sequencing (RNA-Seq). Our transcriptional profiles revealed that numerous differentially expressed genes (DEGs), of which were many more effectors and signaling factors likely involved in morphogenesis and asexual development, multidrug resistance, transcription, transduction, cell rescue, defense and virulence, and carbohydrate metabolism , were significantly down-regulated in expression level by Bmh-delection.

ORGANISM(S): Beauveria bassiana

PROVIDER: GSE58688 | GEO | 2017/06/20

SECONDARY ACCESSION(S): PRJNA253184

REPOSITORIES: GEO

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