RNA-SEQ analysis of single cells extracted from the renal vesicles of P4 Crym-EGFP transgenic mice
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ABSTRACT: We used micro-dissection with FACS sorting techniques to isolate renal vesicle single cell types from post natal day (P4) kidneys. A subset of these single cell populations is analysed individually via Fluidigm single cell analysis. This analysis will determine the transcriptional profile of each cell type, identify compartment specific transcripts, compartment specific transcript isoforms and cell-type specific long-noncoding RNAs. In addition the unbiased nature of RNA-SEQ will potentially identify novel transcripts that have not been annotated in the database.
ORGANISM(S): Mus musculus
PROVIDER: GSE59130 | GEO | 2014/08/01
SECONDARY ACCESSION(S): PRJNA254484
REPOSITORIES: GEO
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