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RNA-seq of IL-4 stimulated human keratinocytes

ABSTRACT: In this study we aim to determine the role of IL-4/STAT6 in gene expression in human keratinocytes using RNA-sequencing approach. Human keratinocytes were cultured for 2 or 5 days with calcium chloride to induce terminal differentiation as determined by the expression of epidermal differentiation complex genes. The cells were then stimulated with IL-4 for 3 and 24 hours, or along the 5 days culture period. We observed that IL-4 inhibits fully differentiation of keratinocytes, induces genes involved with production of inflammatory mediators, and reduces the healing capacity of human keratinocytes. Moreover, STAT6 controlled important genes involved with calcium binding, inflammation and epidermis development.

ORGANISM(S): Homo sapiens

PROVIDER: GSE59275 | GEO | 2015/12/15

SECONDARY ACCESSION(S): PRJNA254943

REPOSITORIES: GEO

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