Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of odorant receptors activated by odorants in vivo

ABSTRACT: Odorants are thought to activate sets of odorant receptors in vivo, but capturing sets of responsive receptors in vivo has never been accomplished. GeneChip microarrays were used to identify the odorant receptor mRNAs enriched in samples from activated olfactory neurons

ORGANISM(S): Mus musculus

PROVIDER: GSE59336 | GEO | 2014/12/02

SECONDARY ACCESSION(S): PRJNA255098

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Molecular profiling of activated olfactory neurons identifies odorant receptors for odors in vivo

Project description:We present a high-throughput in vivo method to identify odorant receptors responding to odorants, using phosphorylated ribosome immunoprecipitation of mRNA from olfactory epithelium of odor-stimulated mice followed by RNA-Seq. pS6-IP RNA-Seq in odor stimulated vs control mice olfactory epithelium

2015-08-24 | E-GEOD-59324 | biostudies-arrayexpress

Molecular profiling of activated olfactory neurons identifies odorant receptors for odors in vivo

2015-08-24 | GSE59324 | GEO

Concentration-dependent recruitment of mammalian odorant receptors

Project description:A fundamental challenge in studying principles of organization used by the olfactory system to encode odor concentration information has been to identify comprehensive sets of activated odorant receptors (ORs) across a broad concentration range inside freely behaving animals. In mammals, this has recently become feasible with high-throughput sequencing-based methods that identify populations of activated ORs in vivo. In this study, we characterized the mouse OR repertoires activated by the two odorants, acetophenone and 2,5-dihydro-2,4,5-trimethylthiazoline, from 0.01% to 100% (v/v) as starting concentrations using phosphorylated ribosomal protein S6 capture followed by RNA-Seq. We found Olfr923 to be one of the most sensitive ORs that is enriched by acetophenone. Using a mouse line that genetically labels Olfr923-positive axons, we provided evidence that acetophenone activates the Olfr923 glomeruli in the olfactory bulb. Through molecular dynamics stimulations, we identified amino acid residues in the Olfr923 binding cavity that facilitates acetophenone binding. This study sheds light on the active process by which unique OR repertoires may collectively facilitate the discrimination of odorant concentrations.

2019-07-31 | GSE135101 | GEO

Mus musculus

Project description:Identification of odorant receptors activated by odorants in vivo

| PRJNA255098 | ENA

Concentration-dependent recruitment of mammalian odorant receptors

Project description:Concentration-dependent recruitment of mammalian odorant receptors

| PRJNA557443 | ENA

Kentucky assay: Citrus accord, 100%

Project description:Mice have 1,100 odorant receptors. Nearly all are orphan receptors. The pattern of receptor responses to odorants is the input that allows the brain to detect and discriminate odors The Kentucky assay measures the function of each of the 1,100 odorant receptors and 14 TAARs in vivo.

2020-03-06 | GSE146417 | GEO

Kentucky assay: Indole/ionone

2020-03-06 | GSE146414 | GEO

Kentucky assay: Indole

2020-03-06 | GSE146413 | GEO

Kentucky assay: bourgeonal

2019-06-01 | GSE123792 | GEO

Kentucky assay: undecanal bourgeonal mixture

2019-06-01 | GSE123791 | GEO