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High-efficiency cellular reprogramming with microfluidics

ABSTRACT: We report that the efficiency of reprogramming human somatic cells to induced pluripotent stem cells (hiPSCs) can be dramatically improved in a microfluidic environment. Microliter-volume confinement resulted in a 50-fold increase in efficiency over traditional reprogramming by delivery of synthetic mRNAs encoding transcription factors. In these small volumes, extracellular components of the TGF-β and other signaling pathways exhibited temporal regulation that appears critical to acquisition of pluripotency. The high quality and purity of the resulting hiPSCs (μ-hiPSCs) allowed direct differentiation into functional hepatocyte- and cardiomyocyte-like cells in the same platform without additional expansion.

ORGANISM(S): Homo sapiens

PROVIDER: GSE59534 | GEO | 2016/04/10

SECONDARY ACCESSION(S): PRJNA255510

REPOSITORIES: GEO

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