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MicroRNA expression profiling during Adipocytic differentiation of human MSCs (hMSC-TERT)

ABSTRACT: We have employed whole microRNA microarray to identify changes in microRNA expression in human bone marrow MSCs (hMSC-TERT) during adipocytic differentiation in culture on day 7 and day 13

ORGANISM(S): synthetic construct Homo sapiens

PROVIDER: GSE59684 | GEO | 2014/09/09

SECONDARY ACCESSION(S): PRJNA255954

REPOSITORIES: GEO

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microRNA expression profiling during Adipocytic differentiation of human MSCs (hMSC-TERT)

Project description:We have employed whole microRNA microarray to identify changes in microRNA expression in human bone marrow MSCs (hMSC-TERT) during adipocytic differentiation in culture on day 7 and day 13 Human MSC line (hMSC-TERT) were subjectd to adipocytic differentiation for 7 days or 13 days, subsequently, RNA was extracted. The samples were labeled using the miRCURY LNAM-bM-^DM-" microRNA Hi-Power Labeling Kit, Hy3M-bM-^DM-"/Hy5M-bM-^DM-" and hybridized on the miRCURY LNAM-bM-^DM-" microRNA Array (6'th GEN). Array product number 208400; Array version 6'th GEN, Array batch number 34014, miRBase version 17. The 'Legends_rawdata.pdf' contains the column headers (of raw data files) and their detailed description.

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Homo sapiens

Project description:microRNA expression profiling during Adipocytic differentiation of human MSCs (hMSC-TERT)

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Gene expression analysis of human bone marrow cells (hMSC-TERT) transfected with mIR-320c or miR-negative control at 72hrs post-transfection

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Gene expression analysis of human bone marrow cells (hMSC-TERT) transfected with mIR-320c or miR-negative control at 72hrs post-transfection

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