Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Ribosome profiling and RNA sequencing of U2OS cells overexpressing myc

ABSTRACT: Ribosome profiling and RNA sequencing was performed on U2OS cells, either control or overexpressing myc.

ORGANISM(S): Homo sapiens

PROVIDER: GSE59819 | GEO | 2017/07/27

SECONDARY ACCESSION(S): PRJNA256317

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Ribo_seq (aka ribosome profiling) analysis of control and Myc-induced U2OS cells

Project description:We used Ribo-seq to examine the effect of Myc activation on protein translation in U2OS cells and correalted these changes with alterations in RNA level measured by RNA-seq on tye same conditions. We also examined these effects in the presence of Torin-1, an inhibitor of mTOR We measure ribosome occupancy profiles in U2OS cells containing an inducible Myc expression vector that were induced or mock-treated in duplicates for 36 hours. In addition, we repeated the experiments in the presence of Torin-1, an inhibitor of mTOR.

2015-11-04 | E-GEOD-66927 | biostudies-arrayexpress

Ribo_seq (aka ribosome profiling) analysis of control and Myc-induced U2OS cells

2015-11-04 | GSE66927 | GEO

GRO-seq analysis of control and Myc-induced U2OS cells

Project description:We used GRO-seq to examine the effect of Myc activation on RNA transcription in U2OS cells. We measure in duplicates gene transcription rates in U2OS cells containing an inducible Myc expression vector that were induced or mock-treated in duplicates for 5 hours.

2015-11-04 | E-GEOD-66928 | biostudies-arrayexpress

GRO-seq analysis of control and Myc-induced U2OS cells

Project description:We used GRO-seq to examine the effect of Myc activation on RNA transcription in U2OS cells.

2015-11-04 | GSE66928 | GEO

RNA-seq analysis of control and Myc-induced U2OS cells

Project description:We used RNA-seq to examine the effect of Myc activation on U2OS cells transcriptome. We also examined these effects in the presence of Torin-1, an inhibitor of mTOR We measure gene expression profiles in U2OS cells containing an inducible Myc expression vector that were induced or mock-treated in duplicates for 36 hours. In addition, we repeated the experiments in the presence of Torin-1, an inhibitor of mTOR.

2015-11-04 | E-GEOD-66789 | biostudies-arrayexpress

RNA-seq analysis of control and Myc-induced U2OS cells

Project description:We used RNA-seq to examine the effect of Myc activation on U2OS cells transcriptome. We also examined these effects in the presence of Torin-1, an inhibitor of mTOR

2015-11-04 | GSE66789 | GEO

Proteome profiling upon Myc activation

Project description:The effect of Myc activation on the proteome was investigated in U2OS cells and proteome changes were combined with Ribo-seq, RNA-seq and GRO-seq analyses.

2015-11-09 | PXD002073 | Pride

Homo sapiens

Project description:Ribosome profiling and RNA sequencing of U2OS cells overexpressing myc

| PRJNA256317 | ENA

Ribosome profiling with MN and RNaseI

Project description:The aim of this experiment is to compare nucleases used in ribosome profiling using Drosophila melanogaster Kc167 cells and human U2OS osteosarcoma cells. Ribosome profiling was performed from nuclease digested samples using sucrose cushion centrifugation separated ribosomes.

2015-01-08 | E-MTAB-2421 | biostudies-arrayexpress

MYC regulates ribosome biogenesis and mitochondrial gene expression programs through its interaction with Host Cell Factor-1

Project description:MYC is an oncoprotein transcription factor that is overexpressed in the majority cancers. Although MYC itself is considered undruggable, it may be possible to inhibit MYC by targeting the co-factors it uses to drive oncogenic gene expression patterns. Here, we use loss- and gain- of function approaches to interrogate how one MYC co-factor—Host Cell Factor (HCF)-1—contributes to MYC activity in a Burkitt lymphoma setting. We identify high-confidence direct targets of the MYC–HCF-1 interaction that are regulated through a recruitment-independent mechanism, including genes that control mitochondrial function and rate-limiting steps for ribosome biogenesis and translation. We describe how these gene expression events impact cell growth and metabolism, and demonstrate that the MYC–HCF-1 interaction is essential for tumor maintenance in vivo. This work highlights the MYC–HCF-1 interaction as a focal point for development of novel anti-cancer therapies.

2020-07-15 | ST001429 | MetabolomicsWorkbench

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data