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Regulation of Activation-Associated MicroRNA Accumulation Rates during Monocyte-to-Macrophage Differentiation

ABSTRACT: We profiled miRNA expression using TaqMan TLDA assays to identify miRNAs with expression levels that were affected by differences in culturing conditions during monocyte-to-macrophage differentiation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE60110 | GEO | 2014/08/06

SECONDARY ACCESSION(S): PRJNA257502

REPOSITORIES: GEO

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Regulation of Activation-Associated MicroRNA Accumulation Rates during Monocyte-to-Macrophage Differentiation

Project description:We profiled miRNA expression using TaqMan TLDA assays to identify miRNAs with expression levels that were affected by differences in culturing conditions during monocyte-to-macrophage differentiation. 9 samples: 3 sample types for 3 different human donors

2014-08-06 | E-GEOD-60110 | biostudies-arrayexpress

Regulation of Activation-Associated Transcripts Accumulation Rates during Monocyte-to-Macrophage Differentiation

Project description:We profiled macrophage activation-associated transcripts using TaqMan assays in a Fluidigm 48.48 Gene Expression Array to identify transcripts with expression levels that were affected by differences in culturing conditions during monocyte-to-macrophage differentiation.

2014-08-21 | GSE60549 | GEO

TaqMan mouse miRNA array profile of LY6CHigh and LY6Clow monocyte

Project description:mouse Freshly isolated monocyte (LY6C high) and (LY6C low) were purified from WT DBA/1 mice blood, and sorted by FACS. We used Taqman miRNA TLDA arrays to performed miRNA profiling

2020-04-10 | GSE137729 | GEO

TaqMan Human miRNA array profile of CD14++CD16- and CD14+CD16++ monocyte subsets

Project description:Human Freshly isolated monocyte (CD14++CD16-) and (CD14+ CD16++) were purified from healthy volunteers' blood, and sorted by FAC. We used Taqman miRNA TLDA arrays to performed miRNA profiling

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Regulation of activation-associated microRNA accumulation rates during monocyte-to-macrophage differentiation.

Project description:Circulating monocytes recruited to tissues can differentiate into macrophages and adopt unique gene expression programs in response to environmental cues. We recently described the regulated expression of several microRNAs (miRNAs) in polarized human monocyte-derived macrophages (MDMs). Basal expression of these activation-associated miRNAs was low in monocytes relative to MDMs. As development occurs in the context of specific cellular environments, we hypothesized that the rate of miRNA accumulation would be modified in the presence of microbial or cellular products during monocyte-to-macrophage differentiation. Indeed, LPS treatment augmented the accumulation of miR-146a and miR-155, whereas IL-4 treatment augmented the accumulation of miR-193b and miR-222 during development. In contrast, some stimuli repressed accumulation of specific miRNAs including interferons (IFNs) (miR-27a, miR-125a-5p, and miR-222), IL-4 (miR-125a-5p), and LPS (miR-27a). RT-PCR-based expression profiling of monocytes differentiated with distinct methods showed that activation-associated miRNAs and markers of macrophage polarization were substantially altered in MDMs differentiated in the presence of non-monocytic peripheral blood mononuclear cells due in part to NF-?B and STAT1 pathway activation. Expression of several of these miRNAs was regulated at a preprocessing step because the expression of the primary miRNAs, but not Dicer, correlated with mature miRNA expression. We conclude that a set of miRNAs is regulated during MDM differentiation, and the rate is uniquely modified for each miRNA by environmental factors. The low basal expression of activation-associated miRNAs in monocytes and their dynamic rates of accumulation during MDM differentiation permit monocytes to tailor miRNA profiles in peripheral tissues during differentiation to macrophages.

| S-EPMC4192495 | biostudies-literature

Monocyte and macrophage lipid accumulation results in downregulated type-I interferon responses

Project description:Monocyte and macrophage lipid accumulation results in downregulated type-I interferon responses

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Steatosis Drives monocyte-derived Macrophage Accumulation in Human Metabolic Dysfunction-Associated Fatty Liver Disease

Project description:Background & Aims: Metabolic dysfunction-associated fatty liver disease (MAFLD) is a common complication of obesity with a hallmark feature of hepatic steatosis. Recent data from animal models of MALFD have demonstrated substantial changes in macrophage composition in the fatty liver. In humans, the relationship between liver macrophage heterogeneity and liver steatosis is less clear. Methods: Liver tissue from 21 participants was collected at time of bariatric surgery and analyzed using flow cytometry, immunofluorescence, and H&E microscopy. Single-cell RNA sequencing was also conducted on a subset of samples (n=3). Intrahepatic triglyceride content was assessed via MRI and tissue histology. Mouse models of hepatic steatosis were used to investigate observations made from human liver tissue. Results: We observed variable degrees of liver steatosis with minimal fibrosis in our participants. Single-cell RNA sequencing revealed four macrophage clusters that exist in the human fatty liver encompassing Kupffer cells (KC) and monocyte-derived macrophages (MdM). The genes expressed in these macrophage subsets were similar to those observed in mouse models of MAFLD. Hepatic CD14+ monocytes/macrophage number correlated with the degree of steatosis. Using mouse models of early liver steatosis we demonstrate recruitment of MdMs precedes KC loss and liver damage and that MdMs may serve a role in lipid uptake during MAFLD. Conclusions: The human liver in MAFLD contains macrophage subsets that align well with those that appear in mouse models of fatty liver disease. Recruited myeloid cells correlate well with the degree of liver steatosis in humans and this occurs prior to changes in KC number. MdMs appear to have a role in lipid uptake during early stages of MALFD.

2023-10-01 | GSE235079 | GEO

Human monocyte-derived macrophage response to Aspergillus fumigatus

Project description:Human monocyte-derived macrophage response to Aspergillus fumigatus

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Global transcriptional profile of hESC-derived macrophage (iMAC), human monocyte-derived macrophage and THP-1 macrophage

Project description:Global transcriptional profile of hESC-derived macrophage (iMAC), human monocyte-derived macrophage and THP-1 macrophage

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Human monocyte derived macrophage microarray analysis

Project description:Microarray data was acquired from human primary monocyte-derived macrophages in order to test the validity of a graphical software package (z-score outlier detection (ZODET)) Complex human diseases can show significant heterogeneity between patients with the same phenotypic disorder. An outlier detection strategy was developed to identify variants at the level of gene transcription that are of potential biological and phenotypic importance. Here we describe a graphical software package (z-score outlier detection (ZODET)) that enables identification and visualisation of gross abnormalities in gene expression (outliers) in individuals, using whole genome microarray data. Mean and standard deviation of expression in a healthy control cohort is used to detect both over and under-expressed probes in individual test subjects. We compared the potential of ZODET to detect outlier genes in gene expression datasets with a previously described statistical method, gene tissue index (GTI), using a simulated expression dataset and a publicly available monocyte-derived macrophage microarray dataset. Taken together, these results support ZODET as a novel approach to identify outlier genes of potential pathogenic relevance in complex human diseases. The algorithm is implemented using R packages and java. 40 volunteer were bled and primary monocyte derived macrophages were cultured. These samples were then randomised into two equal sized groups (control - A1-20 and experimental - B1-20) and run on the ZODET software.

2014-11-05 | E-GEOD-51256 | biostudies-arrayexpress

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