Methylation profiling

Dataset Information

0

Application of a low cost array-based technique - TAB-Array - for quantifying and mapping both 5mC and 5hmC at single base resolution.


ABSTRACT: 5-hydroxymethylcytosine (5hmC), an oxidized derivative of 5-methylcytosine (5mC), has been implicated as an important epigenetic regulator of mammalian development. Current procedures use cost-prohibitive DNA sequencing methods to discriminate 5hmC from 5mC, limiting their accessibility to the scientific community. Here we report a method that combines TET-assisted bisulfite conversion with Illumina 450K DNA methylation arrays for a low-cost high-throughput approach that distinguishes 5hmC and 5mC signals. Implementing this approach, termed TAB-array, we assessed DNA methylation dynamics in the differentiation of human pluripotent stem cells into cardiovascular and neural progenitors. With the ability to discriminate 5mC and 5hmC, we found a much larger number of dynamically methylated genomic regions implicated in the development of these lineages than we could detect by 5mC analysis alone. The increased resolution and accuracy afforded by this approach provides a powerful means to investigate the distinct contributions of 5mC and 5hmC in human development and disease.

ORGANISM(S): Homo sapiens

PROVIDER: GSE60225 | GEO | 2014/12/12

SECONDARY ACCESSION(S): PRJNA257792

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2014-12-12 | E-GEOD-60225 | biostudies-arrayexpress
2019-03-07 | GSE127906 | GEO
2020-09-10 | GSE140125 | GEO
2014-02-14 | GSE46710 | GEO
2014-02-14 | E-GEOD-46710 | biostudies-arrayexpress
2016-02-29 | E-GEOD-63183 | biostudies-arrayexpress
2016-02-29 | GSE63183 | GEO
| PRJNA297146 | ENA
2023-07-14 | GSE236789 | GEO
2023-07-14 | GSE236776 | GEO