Transcriptomics

Dataset Information

0

Identification of novel gene signatures in patients with atopic dermatitis complicated by eczema herpeticum


ABSTRACT: Background: A subset of patients with atopic dermatitis (AD) is prone to disseminated herpes simplex virus (HSV) infection, i.e. eczema herpeticum (ADEH+). Biomarkers that identify ADEH+ are lacking. Objective: To search for novel ADEH+ gene signatures in peripheral blood mononuclear cells (PBMCs). Methods: A RNA-sequencing (RNA-seq) approach was applied to evaluate global transcriptional changes using PBMCs from ADEH+ and AD without a history of EH (ADEH-). Candidate genes were confirmed by qPCR or ELISA. Results: ADEH+ PBMCs had distinct changes to the transcriptome when compared to ADEH- PBMCs following HSV-1 stimulation: 792 genes were differentially expressed at a false discovery rate (FDR) < 0.05 (ANOVA), and 15 type I and type III interferon (IFN) genes were among the top 20 most down-regulated genes in ADEH+. We further validated that IFN-α and IL-29 mRNA and protein levels were significantly decreased in HSV-1 stimulated PBMCs from ADEH+ compared to ADEH- and normal. Ingenuity pathway analysis (IPA) demonstrated that the up-stream regulators of type I and type III IFNs, IRF3 and IRF7 was significantly inhibited in ADEH+ based on the down-regulation of their target genes. Furthermore, we found that gene expression of IRF3 and IRF7 were significantly decreased in HSV-1 stimulated PBMC from ADEH+ subjects. Conclusions: PBMCs from ADEH+ have a distinct immune response following HSV-1 exposure compared to ADEH- . Inhibition of the IRF3 and IRF7 innate immune pathways in ADEH+ may be the important mechanisms for increased susceptibility to disseminated viral infection.

ORGANISM(S): Homo sapiens

PROVIDER: GSE60481 | GEO | 2015/08/18

SECONDARY ACCESSION(S): PRJNA258384

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2016-11-02 | GSE77600 | GEO
2015-04-07 | GSE66486 | GEO
2023-02-16 | GSE182647 | GEO
2015-04-07 | E-GEOD-66486 | biostudies-arrayexpress
2016-03-25 | E-MTAB-3325 | biostudies-arrayexpress
2010-10-15 | E-GEOD-24695 | biostudies-arrayexpress
2020-03-29 | GSE129390 | GEO
2010-10-15 | GSE24695 | GEO
2017-11-07 | GSE104189 | GEO
2024-03-26 | GSE262071 | GEO